Volume 25, Issue 3, Spring 2014, Page 1-109

Detection of the Predominant Strain of Epstein-Barr Virus in Systemic Autoimmune and Thalassemia Patients

Basima A. Abdullah; Alaa Y. Al-Hamadany

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 1-9
DOI: 10.33899/rjs.2014.88563

EBV, is a member of the herpesvirus family and one of the most common human viruses, Epidemiological data suggest that EBV is associated with polytransfused blood β-thalassemia and several autoimmune diseases, such as systemic lupus erythematosus, rheumatoid arthritis and multiple sclerosis. We examined the presence of IgM antibodies against EBV in serum of 35 Thalassemic patients, 75 autoimmune patients among as 35 rheumatoid arthritis patients, 20 Systemic lupus erythrematosus and 20 autoimmune hypothyroid diseases, and 20 healthy controls by ELISA assay then detected the predominant strain in positive samples. The results show that the highest EBV infection percent was in SLE 15% whilst the lowest infection percent was in Thalassemia 5.7%., and according to gender, the results showed that the highest infection percentage recorded in females with rheumatoid arthritis 30 %, whilst the infection does not appear in males with rheumatoid arthritis and autoimmune thyroid disease and females of thalassemia patients. On the other hand, this study reveals that EBV-1 is the predominant strain in autoimmune diseases and thalassemia in Iraq.

Development of Spectrophotometric Method for the Determination of Metoclopramide. HCl in a Pharmaceutical Preparations

Rowa N. Aljarah; Aseel N. Obedagha

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 10-21
DOI: 10.33899/rjs.2014.88564

This paper includes a development of spectrophotometric method for the determination of metoclopramide.HCl. The method involves the diazotization of metoclopramide.HCl and coupling with pyridoxine. HCl, to form an intense orange colored, water-soluble and stable azo-dye which shows a maximum absorption at 470 nm. Beer's law is obeyed over the range 0.5-30 µg/ml metaclopramide. HCl with a molar absorptivity of 1.98*104 L.mol-1.cm-1, The average recovery is 99.27% and relative standard deviation of  0.124 to  0.778%. This method has been applied successfully to the determination of metoclopramide.HCl in pharmaceutical preparations.

The Removal of Lead, Copper and Zinc from Industrial Wastewater Using Grape Fruit Peels

Sufyan M. Shartooh; Hussain Sh. Gabur; Hussain Sh. Gabur

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 22-31
DOI: 10.33899/rjs.2014.88565

The current study was designed for using grape fruit peels (Citrus paradise) to remove lead, copper and zinc from industrial wastewater. Three forms of these peels (fresh, dried small pieces and powder) were tested under some environmental factors such as pH, temperature and contact time. Current data show that grape fruit peels are capable of removing lead, copper and zinc ions with a significant capacity. Furthermore, the powder of grape fruit peels had a highest capability in removing all lead, copper and zinc ions followed by fresh peels whilst dried peels had the lowest bioremoving capacity again for all metals under test. The highest capacity was for lead then copper and finally zinc. All these data were significantly (LSD peel forms = 3.349 mg/l) varied. However, some examined factors were found to have significant impacts upon the bioremoval capacity of grape fruit peels such as pH, temperature, and contact time where best biosorption capacity was found at pH 4, at temperature 50 Cº and contact time of 1 hour. It is true that grape fruit peels varied significantly in case of metal ions and increasing examined factor (pH, temperature, and contact time). The fourier transform infrared spectroscopy analysis (FTIR) illustrated that hydroxyl, carboxyl and carbonyl groups were the major binding sites for Pb, Cu and Zn ions by grape fruit peels.

U.V. Spectroscopic Studies of the Schiff Base N-(o-N-(Methyl-3-ol-2-butenimino)- -(o-Toluyl)-1,2- diphenylethandimine and its Complexes with Co(II), Ni(II), Cu(II) and Pd(II): Direct Determination in Absolute Ethanol

Amel G. Abed

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 32-43
DOI: 10.33899/rjs.2014.88566

The U.V. Spectra were used for the direct quantification of the Schiff base N-(o-N-(methyl-3-ol-2-butenimino)- -(o-Toluyl)-1,2-diphenyl-ethandimine and its complexes with Co(II), Ni(II), Pd(II) and Cu(II) in absolute ethanol at = 344 nm for the Schiff base, Co(II), Ni(II) and Pd(II) complexes and at  = 260 nm for the Cu(II) complex. The direct determination limit was estimated through the plot of the molar concentration against the absorbance and R2 and R.S.D for each complex were calculated. The method appears to be in a good accuracy and precision.

Spectrophotometric Determination of Thymol in Pharmaceutical Preparation via Diazotization Reaction with 4-aminoacetophenone

Salih T. Al-Ramadhani

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 44-56
DOI: 10.33899/rjs.2014.88567

A simple and sensitive spectrophotometric method is proposed for the determination of thymol. The method is based on the coupling reaction of the drug with diazotised 4-aminoacetophenone reagent in alkaline medium to produce an intense red coloured water -soluble and stable azo dye which exhibits a maximum absorption at 502 nm. Beer’s low is obeyed over the concentration range 0.2 – 16 µg/ml with a molar absorptivity of 2.89×104 L.mol-1.cm-1. The limit of detection is 0.0216 µg/ml and the limit of quantitation is 0.072 µg/ml, average recovery is 101.6%, and RSD is less than 2.4%. The proposed method is applied successfully to the determination of thymol in pharmaceutical preparation.

Synthesis and Characterization of 2-Butoxyethylxanthate Complexes with Iron(II), Cobalt(II), Nickel(II), Copper(II) and Zinc(II) and their Adducts with Nitrogen Base Ligands

Ibraheem A. Al-Qasser; Saad E. Al-Mukhtar; Nada F. Hana

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 57-64
DOI: 10.33899/rjs.2014.88644

New complexes and adducts of xanthate of the general formula [M(2-BuoEtxant)2] and [M(2-BuoEtxant)2.nL] Where M= Fe(), Co(), Ni(), Cu() and Zn(), and (2-BuoEtxant)=2-Butoxyethylxanthate, and n=2 when L= pyridine; Isoquinoline, γ-picoline, 3,5-lutidine, n=1 when L= ethylenediamine, (1,10)-phenanthroline, have been prepared and characterized by metal analyses, infrared, conductance measurements, electronic absorption spectra and susceptibility measurements. Magnetic moment and electronic spectra, indicate that the complexes of type [M(2-BuoEtxant)2] are of tetrahedral geometry while the complexes of type [M(2-BuoEtxant)2.nL] have an octahedral geometry.

Synthesis and Characterization of Mn(II), Co(II), Ni(II) and Cu(II) Complexes with New Schiff Base Derived from Isophthalaldehyde and 2-amino-5-nitropyridine

Widad T. Al-Kattan; Esraa M. Al-Nidaa

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 65-73
DOI: 10.33899/rjs.2014.88645

A series of new complexes of the type [M2LCl4] and [M2L2Cl4] where M=Mn(II), Co(II), Ni(II) and Cu(II), L={1,3-Bis(2-amino-5-nitropyridine)isophthylidene} have been prepared. Adduct complexes of the type [M2L(PPh3)2Cl2]Cl2 were M= Co(II) and Ni(II) were also prepared in 1:2 (complex: PPh3) molar ratio. The complexes were characterized by molar conductance, magnetic moment, and FT-IR, UV-visible spectral studies and metal content analysis. Conductivity data showed that the complexes were non-electrolytes and the PPh3 adducts were 1:2 electrolytes. Magnetic moments and electronic spectra indicate that the complexes have either tetrahedral or octahedral geometry while the complex Ni2LCl4 has square planer configuration.

The Effect of Annealing on Nano-Topography of SiO2 Film

Assim A. Issa

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 74-86
DOI: 10.33899/rjs.2014.88647

This research studies the effect of annealing in temperature range (300 - 600)°C on nano-topography of SiO2 film. SiO2 nano film growth on Si (100) p-type substrates, by using the anodic oxidation technique using (%75H2O+%25 isopropanol) solution containing 0.1N KNO3 as supporting electrolyte and applied potential is 5 volts. The chemical analysis of the surface of SiO2 has been done by (EDAX) shows the presence of O and Si elements. (AFM) is used to study the nanotopography of SiO2 nano film. However, it has been found that all of the following characteristics, root mean square RMS surface roughness of the SiO2 film, grain area, grain volume and grain length increase with the increase of annealing temperature.

Density and Mass Distribution of the Spiral Galaxy NGC 3672

Nazzeha A. Daod; Mohammed K. Zeki; Aziz J. Abdul-Kahader

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 87-94
DOI: 10.33899/rjs.2014.88648

The density and mass of Spiral galaxy NGC 3672 has been studied as a function of the distance from the center of the galaxy. The mass of the three regions of the galaxy (nucleus, disk and halo) have been calculated and compared with other work. The total mass of NGC 3672 is found to be 1.22×1011 Mo at a radius of 23.7 kpc.

Comparative Study for Temperature Dependence of Bulk Modulus for Solids: Pyrope Garnet as an Example

Khaled S. Hameed

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 95-100
DOI: 10.33899/rjs.2014.88650

A comparative study for temperature dependence of bulk modulus and an investigation for the evolution of this variation have been established, by using five different theoretical equations, according to their historical development. The theoretical results obtained in this work have been compared with experimental data. Pyrope garnet, Mg3Al2(SiO4)3, has been chosen as an example for solid materials.

A Study of the Thermal Neutron Effects on the Optical Properties of CR-39 Solid State Nuclear Track Detector

Sheamaa T. Al-Dbag

Rafidain Journal of Science, 2014, Volume 25, Issue 3, Pages 101-109
DOI: 10.33899/rjs.2014.88651

This research involves studying the effect of thermal neutron on the optical properties of nuclear track detector (CR-39) exposed to radiation at different time ranges from (1 to 15) day at room temperature. The photo absorption was measured using a spectrometer (UV-1100). The results show that the coefficient of absorption increases by increasing time of neutron flux exposure. It was found that the energy gap values decrease with the increase of exposure radiation time, the results also show that the value of energy gap at direct effect (before and after) radiation is more than its value in the indirect effect, also the numbers of carbon atoms in a cluster were determined from UV-visible spectra of the thermal neutron irradiated samples.