Volume 24, Issue 10, Autumn 2013, Page 1-98

Isolation and Identification of Bacteria from Genital Organs of Pigeons

Ebtehal N. Mahmmoud

Rafidain Journal of Science, 2013, Volume 24, Issue 10, Pages 1-15
DOI: 10.33899/rjs.2013.78799

This study includes an examination of 70 bacterial swabs taken from ovary, oviduct of femal and male testis from adult pigeons of species Collard dove (Streptopelia decaocto), from December 2010 to June 2011. The pigeons were clinically healthy. The isolates were 12 species and 21 types of bacteria. The type Staphylococcus xylosus was highest ratio (11.7%) and types Micrococcus luteus and Gallibacterium anatis were lowest ratio (1.8%). Other isolates of bacteria were distributed between these two percentages. Regarding the isolate site, the highest isolation ratio of ovarian swabs was Enterococcus durans and Bacillus subtilis (12%), while the higher bacteria ratio isolated from the oviduct swabs are En. avium, Escherichia coli and Pasteurella multocida (9%), the testicular swabs were Staph. xylosus most isolation ratio (20%), while isolate each of species Pseudomonas aeruginosa, Staph. xylosus, Staph. saprophyticus, En. faecalis and E. coli of swabs taken in the case of ovulation from female pigeons, has also investigated some of the Virulence factors which are possessed by these isolates such as Urease, DNase, Gelatinase and Haemolysin, the study found that these isolates have some of these factors.

Effect of some Kinds of Fungal and Bacterial Biopesticides for the Control of Damping-off and Root Rot Fungi of Okra Seedlings in the Greenhouse

Najwa B. Al-Lashi

Rafidain Journal of Science, 2013, Volume 24, Issue 10, Pages 16-37
DOI: 10.33899/rjs.2013.78801

Fungal biopesticides preparations Trichoderma harzianum, T.viride individually or in combination with the bacterial biopesticides Pseudomonas fluorescens or Bacillus subtilis showed a significant inhibition to the mycelial growth of fungi causing damping-off and root-rot of okra seedlings Abelmoschus esculentus L.. The maximum inhibition of the growth of fungal colonies was recorded with a mixture of Trichoderma harzianum + Pseudomonas fluorescens which caused complete inhibition (100%). Seed treatment with a mixture of biopesticides proved to be superior than individual treatments in reducing the infection of damping –off disease significantly and increasing the length of shoots and roots and dry weight of the plant. The use of biofungicide preparations individually or in mixture raised the levels of peroxidase and polyphenol oxidase enzymes indicating the induction of systemic resistance of okra plants against fungi causing damping- off and root-rot disease. A highest enzyme activity was noticed with Trichoderma harzianum + Psedomonas fluorescens in soil contaminated with F.solani or M. phaseolina and reached 2.823 and 0.185/min./g fresh weight respectively.

Spreading of Enterococcus faecalis and Enterococcus faecium in Hospitals and Water Environment in Mosul City

Muhsin A. Essa; Hala Z. Najem

Rafidain Journal of Science, 2013, Volume 24, Issue 10, Pages 38-54
DOI: 10.33899/rjs.2013.78803

This study was performed to determine the prevalence of Enterococcus faecalis and Enterococcus faecium in hospitals and water environment in Mosul city. 240 specimens were collected during the period from September to December / 2011, 120 specimens were from water environment in addition to 120 specimens from hospitals environment (General Hospital, Ibin-Sena Hospital and Al-Khansaa teaching Hospital). Isolation and identification were done using a number of specific selective media for Enterococcus in addition to biochemical and serological tests then conformational tests were done using API 20 Strep and ID32 Strep.
The results illustrated that the genus Enterococcus was isolated at 30% of all isolates (56.94 % was from Hospitals and 43.05% was from water environment). The isolated ratio of E. faecalis (18.1%) was higher than E.faecium (12.5%).

Estimation the Level of IL-6 in Pregnant Women Suffering from Urinary Tract Infections

Abeer H. Al-Khafaf; Adeeba Y. Shareef

Rafidain Journal of Science, 2013, Volume 24, Issue 10, Pages 55-63
DOI: 10.33899/rjs.2013.78805

The study included the isolation of bacteria causing Urinary Tract Infection in pregnant women, and determine the level of IL-6in pregnant women with Urinary Tract Infection, and comparing it with healthy pregnant women and healthy non-pregnant women in Nenevah Provence. (200) urine samples from UTIs infected pregnant women, their ages ranged between (15-47) years were collected, The results showed UTI bacterial infection in pregnant women at a rate of (43.5%). The level of IL-6 was determined on (66) serum samples of UTI infected women, (10) healthy pregnant and (10) samples of healthy non-pregnant women, the results showed a decrease in the level of IL-6 in the pregnant women having UTI and the healthy pregnant, compared with the healthy non-pregnant women. (69.456 ± 39.132, 72.163 ± 36.417, and 127.302 ± 44.575) pg/ml for the three groups respectively. and no variation was detected in its level during the stages of pregnancy.

Biochemical Study of Ribonuclease in Serum and Tissues of Patients with Uterine Tumors

Saba Z. Al-Abachi; Duaa H. Omar

Rafidain Journal of Science, 2013, Volume 24, Issue 10, Pages 64-80
DOI: 10.33899/rjs.2013.78809

The activity of alkaline and acidic ribonuclease (RNase) was measured in serum and tissues of women with benign and malignant uterine tumors, compared to women with a curettage as a control group. The results indicated that there is a significant increase in alkaline and acidic RNase activity in sera and tissues of patients with uterine tumors when compared with the control group.
The research also, includes the isolation and purification of the alkaline RNase from the sera of normal women. It has been found that the specific activity of enzyme in proteinous precipitate, after dialysis and gel filtration, was increased and the enzyme shows two peaks. In addition, it has been found that the enzyme has approximate molecular weight of (28774±1000) dalton.
The results also showed that the optimum conditions of the RNase enzyme are: (50) µg/ml of enzyme concentration with reaction (15) minutes at (pH= 8.2) and at (37˚C), while the substrate concentration was about (0.125) mmol/L. When the Lineweaver-Burk plot was used, the value of Michaelis-Mentens constants (Km) is (0.06 mmol). When the duration of storage was studied, the activity of the enzyme is not affected during two months, after that the activity was decreased gradually.

Study of Austenitic Alloy AISI304 Oxidation after Coating by Aluminum and Chromium at High Temperature

Yahya A. AL-Salman; Mahmood A. Hmood; Ahmad N. Mahmood

Rafidain Journal of Science, 2013, Volume 24, Issue 10, Pages 81-90
DOI: 10.33899/rjs.2013.78811

The present research study the effect of metallic coating namely aluminizing and chromizing on the oxidation resistance of stainless steel alloy (AISI 304). The oxidation kinetics of a single stage coating such as Aluminized and Chromized alloy have been studied under atmospheric pressure at 1000 ˚C using a thermal cyclic oxidation then compared with uncoated alloy. The results show, the two samples of coatings such as aluminized and chromized which have a two different resistances of cyclic oxidation, two results show of three samples stainless steel alloy AISI 304 and aluminum, chromium coatings, the reduction in the weight after 225 (hr) is about (5.81-0.3-0.01)gm/cm2 successively. aluminized coating exhibited the greatest resistance to oxidation. The superiority protection of aluminized coating can be due to the formation of Al2O3 and improving scale via pegs formation.

Using Heat Sinks in Thermal Cooling Solar Cells and its Effect on Cell Performance

Huda M. Abd Al-Qader

Rafidain Journal of Science, 2013, Volume 24, Issue 10, Pages 91-98
DOI: 10.33899/rjs.2013.78813

In this research, a heat sink which is normally used to cool electronic devices, integrated circuits and microprocessors, is used to cool a solar cell and study the effect of this cooling method on solar cell temperature and performance. We use a halogen lamb as a light and heat source. The results show that when solar cell temperature increases, the open circuit voltage of solar cell decreases rapidly, but when we use the heat sink, the temperature increases slower at the same time of duration therefore the open circuit voltage decreases slower than the first case. This means that the solar cell will work at a higher efficiency under the same condition.