Print ISSN: 1608-9391

Online ISSN: 2664-2786

Volume 23, Issue 6

Volume 23, Issue 6, Summer 2012, Page 1-179


Measurement of Specific Activity of Dihydrofolate Reductase (DHFR) Enzyme and Determination of Folate Content Extracted from UV-Exposed Stem- Derived Callus of Sunflower (Helianthus annuus L.)

Sajida A. Abood; Firas H. Khathyer

Rafidain Journal of Science, Volume 23, Issue 6, Pages 1-11
DOI: 10.33899/rjs.2012.59598

The results revealed to the increase of the specific activity of dihydrofolate reductase in stem' callus of sunflower, Heliathus annuus L. exposed to UV(360 nm.) for 5,10 and 15min. with the growth period. The total content of folate increased substantially, whereas 20min decreased activity of dihydrofolate reductase and folate content extracted from callus of different age.
The inhibitory effect of folate analogoues aminopterin and trimethoprim, In Vivo and In Vitro on dihydrofolate reductase and folate content of sunflower callus exposed or nonexposed to UV was tested. The results revealed that the exposure of callus for five min. exhibited high level of resistance to trimethoprim and aminopterin. The specific activity of dihydrofolate reductase and the total content of folate partially reserved as a result of radiation compared with non-radiated callus

Isolation and Identification of Brevibacterium Species from Dairy Product and some Clinical Specimens

Nadia H. Waad Al-Hyally; Essra G. Hazem Al-Sammak

Rafidain Journal of Science, Volume 23, Issue 6, Pages 12-26
DOI: 10.33899/rjs.2012.59600

Isolation and identification of the member of the genus Brevibacterium which belong to Actinomycetes, were carried out, a total of (175) sample from different sources of dairy products, human blood and skin from kidney dialysis and cancer patient from August 2010 to February 2011.
The result indicate the higher percentage of isolation from dairy (32%), cheese and milk (20%) for each of them, blood of kidney dialysis 12% (8% male and 4% female)and from skin of kidney dialysis patient (4%), and they didn’t isolated from skin and blood of cancer patient.
The isolates were purified on the modified media Plate Count Agar (PCA) and blood agar and was purified and then identified to species level depending on the morphological, characteristics as shape, color and consistency of colony, sensitivity to antibiotics, most of species have shown sensitivity to Ciprofloxacin 5Mg/disc and resistance to penicillin and ampicillin 10Mg/disc, in addition to biochemical and physiological tests to the following
species B. linens, B. epidermidis, B. otitidis, B. iodinum, B. casei, B. sanguinis and
B. paucivorans.

Estimation of Heterosis and Heritability in Bread Wheat (Triticum aestivum L.)

Raed S. A. Al-Saffar; Zahraa K. H. Al-Sawaf

Rafidain Journal of Science, Volume 23, Issue 6, Pages 27-38
DOI: 10.33899/rjs.2012.59603

Six varaieties of bread wheat Triticum aestivum L.(Sham 4, Adnaniya, Iba 95, Tamoz 2, Eratom, Abo-Graib) and their half diallel crosses were used to estimate heterosis, broad and narrow sense heritability for the following quantitative characters: heading time, number of tillers per plant, plant height, flag leaf area, number of spikes per plant, spike length, number of spiklet per spike, number of grains per spike, 100 grain weight, grain yield per plant and the harvest index. Most hybrids appeared significant and desirable values for heterosis. The broad sense heritability was high for plant height, spike length and number of spiklet per spike while, it was modrate for harvest index, and low for heading time, number of tillers per plant, flag leaf area, number of spike per plant, number of grains per spike, 100 grain weight and grain yield per plant. The narrow sense heritability was high for plant height and harvest index, and moderate for heading time, spike length, number of spiklet per spike,100 grains weight while it was low for number of tillers per plant, flag leaf area, number of spike per plant, number of grains per spike and grain yield per plant.

Study of the Effect of Zinc on the Fertility and Acid Phosphatase Activity and Fructose Concentration in Male Swiss Albino Mice ( Mus Musculus)

Tamara W. Jihad

Rafidain Journal of Science, Volume 23, Issue 6, Pages 39-54
DOI: 10.33899/rjs.2012.59604

This study was conducted to elucidate the effect of different concentrations of zinc element on male fertility of swiss albino mice by using atomic absorption, and determination of acid phosphatase activity ( Ec.3.1.3.2) compared with control groups. The mice were divided into three groups ( 10 mice / group ) and they were three months old. The first group was treated with 0.5 mg / kg body weight and the second group with 1.5 mg / kg body weight with zinc, while the third group considered as control group. The mice treatment was for 15 and 30 days for the two experiment groups.
The results showed a significant increase ( p≤0.05 ) in weight of testes in the group which treated by concentration 1.5 mg / kg b.w compared with control group. Significant increase (p≤0.05) was also found in testes weight when using the high concentration of zinc compared with the low concentration. Results also showed a significant increase in sperm count of groups treated with zinc as compared with control group. Significant increase (p≤o.o5) in sperm motility was detected in both the first and second groups as compared with control . Significant decrease (p≤0.05) in slugish sperm in group with high concentration of zinc as compared with control. Significant increase in both groups in acid phosphatase activity and the concentration of fructose treated with concentrations 0.5 and 1.5 mg / kg b.w as compared with control.

Effect of Cholesterol and Boiled Extract of Red Pepper on the Level of some Hormones and Weights of Local Male Rabbits

Muntaha M. Al-Kattan; Hadeel T. Al-Ishlash

Rafidain Journal of Science, Volume 23, Issue 6, Pages 55-67
DOI: 10.33899/rjs.2012.59608

The current study was designed to investigate the effect of cholesterol supplementation at a dose of (260 mg/kg ration) and boiled extract of red pepper at a dose of (100 mg/kg body weight) on the Testosterone hormone, Thyroid stimulating hormone or Thyrotropin, thyroxine, thyronine, and it is effects on the body weights of male rabbits after 4, 8 weeks of the start of treatment.
Forty local male rabbits aged (8-10) months old were randomly divided into four groups (10 animals/ group) to be treated as follows:
First group: Standard ration and distilled water represented as control groups.
Second group: Standard ration plus cholesterol at the dose (260 mg/ kg ration) and distilled water.
Third group: Standard ration with drinking red pepper fruit boiled extract at the dose (100 mg/ kg body weight).
Fourth group: Standard ration plus cholesterol (260 mg / kg ration) in addition to drinking red pepper fruits boiled extract at a dose of ( 100 mg/kg body weight).
All groups were treated daily for eight weeks.
Results showed that cholesterol treatment caused significant decrease(P≤0.05) in the hormones level, with a significant increase (P≤0.05) on the body weights of treated rabbit after 4 and 8 weeks. Furthermore, treatment with red pepper boiled extract led to a significant increase (P≤0.05) on the levels of the studied hormones, and a significant decrease (P≤0.05) in the body weights of treated rabbits.

Genetical Analysis of Self-Fertilized Generation Variances in Two Durum Wheat Crosses

Nabeel T. Al-Badrany; Najeeb K. Yousif; Ghada A. Al-Hamdany

Rafidain Journal of Science, Volume 23, Issue 6, Pages 68-82
DOI: 10.33899/rjs.2012.59623

Phenotypic variance in parents and third generation were analyzed to estimate the additive genetic variance, dominance genetic variance, environmental variance, average degree of dominance, heritability in broad sense, heritability in narrow sense and expected genetic advance from selection in F3 generation, of two crosses in durum wheat (Triticum durum Desf.), the first cross between Azeghar-1 and Um-Rabie-5 and the second cross between Leeds and Waha for traits, heading time, flag leaf venation, plant height, peduncle length, number of spike, spike length, grain yield, 100 grains weight and number of grains per spike. Average degree of dominance revealed the different types of dominance for the studied traits, the values of narrow sense heritability were high for plant height, peduncle length, number of spikes, grain yield and number of grains per spikes in both crosses, spike length and weight of 100 grains in first cross, and flag leaf venation in second cross.

Detection of the Occurrence of Antibiotic Residues in Different Kinds of Milk

Aytham S. AL-Dabbagh

Rafidain Journal of Science, Volume 23, Issue 6, Pages 83-92
DOI: 10.33899/rjs.2012.59625

The study detected the occurrence of antibiotic residues in (187) milk samples. The samples included 114 fresh raw milk of cows, buffaloes, ewes and goats and 73 milk samples of pasteurized liquid and dried powder intended for adult and for infant consumption, The samples were randomly collected from different regions and markets in Mosul city. The antibiotic residues were detected in 38.9%, 9.1% , 33.3% , 30 % in milk samples of cows, buffaloes, ewes, and goats, respectively. Antibiotic residues were found in 23.5% of pasteurized milk, and 23.3% in adult milk and 15.4% in infant milk. Results showed a significant difference, in the occurrence of antibiotic residues between buffalo milk and cow, ewes and goats milk, and between infant and adult milk and also with pasteurized milk.

Genetical Analysis of Self-Fertilized Generation Means in Two Durum Wheat Crosses

Nabeel T. Al-Badrany; Najeeb K. Yousif; Ghada A. Al-Hamdany

Rafidain Journal of Science, Volume 23, Issue 6, Pages 93-106
DOI: 10.33899/rjs.2012.59631

Six self-fertilized generations (P1, P2, F2, F3, B1S and B2S) of two crosses in durum wheat (Triticum durum Desf.), the first cross between Azeghar-1 and Um-Rabie-5 and the second cross between Leeds and Waha, were used to study gene action for the traits, heading time, flag leaf venation, plant height, peduncle length, number of spikes, spike length, grain yield, 100 grains weight and number of grains per spike. Two models were used in analyzing the components of the generation means, three –parameter model and six-parameter model. The results from the generation mean analysis showed that the three- parameter model was adequate for the inheritance of spike length and weight of 100 grains in the both crosses and number of spikes per plant in the second cross but there were additive, dominance and epistatic effects on the other characters in the both crosses.

Biochemical Study of Myeloperoxidase in Blood and it's Relation to Atherosclerosis

Thikra A. Allwsh; Mahmoud A. M. Fakhri; Mohammad A. Hamza

Rafidain Journal of Science, Volume 23, Issue 6, Pages 107-127
DOI: 10.33899/rjs.2012.59635

The research included studying of myeloperoxidase in human serum, The normal value of myeloperoxidase activity was found (27.71±0.74 U/ml) in control group for both sexes at (19-70 year), with no sex difference, while it was affected by age and smoking. The study also showed a significant increase in myeloperoxidase activity (38.95±1.01 U/ml) in serum of atherosclerosis patients group for both sexes at (31-80 year) in comparison with control, on the other hand, myeloperoxidase activity in serum of patients group was affected by sex, age, smoking and the intake of statins.
The results also indicated a significant increase in the concenteration of total cholesterol, low density lipoprotein -cholesterol, C-reactive protein and troponin. While a significant decrease in high density lipoprotein -cholesterol, total protein, albumin, globulin, chloride and arylesterease in serum of patients group in comparison with control. Correlation study was performed between the measured biochemical parameters and myeloperoxidase activity in serum by linear correlation coefficient, The results also showed that there was a significant positive correlation between myeloperoxidase activity and troponin in atherosclerosis patients.
Also, the research included the isolation of myeloperoxidase from normal human serum using different biochemical techniques, including: precipitation by ammonium sulfate, dialysis, gel filtration chromatography on sephadex G-100. The results predicted that spicefic activity and the number of fold of purification were (8.865 U/ml) and (25) respectively for partially purified enzyme. Furthermore, the comparative molecular weight of the partially isolated myeloperoxidase was (151±1.8 kDa) using gel filtration chromatography. The results were predicted also that myeloperoxidase containing heam group, zinc and calcium elements, which indicated that the enzyme is glycoproteins type.
The study showed that the optimum conditions of myeloperoxidase were obtained at the first minute using sodium citrate (0.1 M) as buffer at pH (5.0), at a temperature (45 ºC) and (12 mM) of ο-dianisidin as substrate. It was found that Vmax and Km have the values of (24.27 U/ml) and (2.443 mM) respectively.
Ethylene diamine tetraacetic acid, sodium azide, 2-mercaptoethanol and sodium chloride showed inhibition on the activity of myeloperoxidase, where sodium azide had more inhibition in the activity and in an irreversible non competitive inhibition type.

Clinical Study of Myeloperoxidase and some Biochemical Parameters in Diabetes Mellitus Patients

Mahmoud A. M. Fakhri; Zuzan E . Mahmood

Rafidain Journal of Science, Volume 23, Issue 6, Pages 128-145
DOI: 10.33899/rjs.2012.59637

The research included a clinical studying of Myeloperoxidase enzyme and some biochemical parameters in serum. The normal value of Myeloperoxidase activity in control group was (30.27 U/ml) for both sexes in age group (15-77 year). The results showed that Myeloperoxidase activity in the serum of control group was not affected by sex and marital status, while it was affected by age, smoke and body mass index.
The study also showed a significant decrease in serum Myeloperoxidase activity of Diabetes Mellitus patients group (19.17 U/ml) for both sexes at in age group (31-80 year) comparison with control, and the enzyme activity in serum of patients group was not affected by sex, marital status and type of disease, while it was affected by age, smoke and body mass index.
The results indicated a significant decrease in serum Myeloperoxidase activity in type Ι, type Π Diabetes Mellitus male in comparison with control male, and enzyme activity in type Ι, type Π Diabetes Mellitus female in comparison with control female. Also the results showed a significant decrease in enzyme activity in age groups of type Ι, type Π patients in comparison with age groups of control, so that in comparison the body mass index for type Ι patients with control. The results also predicted a significant decrease in serum Myeloperoxidase activity in type Ι, type Π Diabetes Mellitus smokers in comparison control smokers, and nonsmokers in comparison control nonsmokers.
The study also showed a significant increase in the level of Glucose, Total Cholesterol and Low Density lipoprotein, while a significant decrease in the level of Total protein, Albumin, Globulin and High Density lipoprotein in serum of type Ι Diabetes Mellitus patients in comparison control. Also noticed a significant increase in the level of Glucose, Triglyceride, Total Cholesterol, Low Density lipoprotein, Very Low Density lipoprotein, while a significant decrease in the level of Total protein, Albumin, Globulin and High Density lipoprotein in serum of type Π Diabetes Mellitus patients in comparison control.

The Effect of Macroscopic Texturing in one Dimension on the Characteristics of Amorphous Silicon Solar Cells

Shema Kadir Ismeal; Bassam M. Mustafa

Rafidain Journal of Science, Volume 23, Issue 6, Pages 146-162
DOI: 10.33899/rjs.2012.59639

In this research studied the effect of macroscopic texturing on the characteristics of amorphous silicon solar cells. The texturing is in the form of long grooves with shape V and of groove angles (acute, right, obtuse) (40ْ, 90ْ, 110ْ ) a theoretical method is found to calculate the increase of photo current due to increase of optical energy trapped by texturing. Our study concentrate on the case when the solar panels exist in its practical situation and for the coordinates of Mosul city for the four season of the year. For acute angles there is no increase of efficiency, but for the right and obtuse angle the efficiency reach 12 and 14 % .

Study the Physical Properties Antimony Doped Zinc Oxide Thin Films Prepared by Chemical Vapor Deposition

Basil H. Nasir; Mekhaiel I. Manssor

Rafidain Journal of Science, Volume 23, Issue 6, Pages 163-179
DOI: 10.33899/rjs.2012.59641

Undoped ZnO and Sb-doped ZnO (0.3-10 at. %) thin films have been prepared by APCVD technique on the glass substrate at (400Cº, 425Cº, 450Cº, 475Cº, 500Cº(. The structural, optical and electrical properties of these thin films were studied. The results of the structural tests showed that these Films of ZnO:Sb were successfully prepared by (APCVD) X-ray measurement revealed that thin film structure was Polycrystalline of Hexagonal Wurtzite type with preferential orientation along the (002), (100) direction, in addition peaks for some phases for Antimony oxides were appeared. AFM measurement revealed that thin film have Roughness Average (19.4 nm) & RMS (24.3 nm). The optical measurement Transmission (T') were increase with as the doping percentage increased and decrease with increased substrate temperature, were found (79-90%), the band gap energies of the ZnO:Sb thin film are nearly the same as the pure ZnO. the best Electrical conductivity is appear with ZnO thin and (10% Sb) concentration at substrate temperatures (400Cº). The Hall measurement revealed that Resistivity were (1.8 Ω.cm), mobility ((146-4749) cm2/V.sec) and carrier concentration (1015 cm-3). The doped and undoped ZnO films exhibited n-type conductivity.