Print ISSN: 1608-9391

Online ISSN: 2664-2786

Volume 21, Issue 1

Volume 21, Issue 1, Winter 2010, Page 1-100

Detection of E.coli O157:H7 Strain Among Bacteria Contaminated Drinking Water in Nineveh Province

Angham J. A. Al-Oqaidy; Muhsin A. E. Al-Oqaidy

Rafidain Journal of Science, Volume 21, Issue 1, Pages 1-15

This research, was dealt with the isolation and identification of bacteria caused contamination in drinking water, and detection on the virulent pathogen E. coli O157: H7 strain in contaminated drinking water samples, the results indicated that most isolated bacteria belong to Enterobacteriaceae and the higher percent was E. coli (%32) , in addition to other bacteria ,found among there were Enterobacter cleacae (%19.3), E. sakazakii (%5.9), Citrobacter braakii (%8.8), Citrobacter freundii (%3.0), Proteus mirabilis (%7.4), Shigella sonni (%0.7), Salmonella (%5.2), Serratia plymuthica (%1.5), pseudomonae aeruginosa (%5.2) and Enterococcus faecalis (%10.4) .
E. coli serotypes were diagnosed in drinking water samples, to detect O157: H7 strain the result showed that (47.7 %) from all E. coli strains and (%15.6) from all other bacteria, while O157 strain formed (%22.7) percent and (%7.40) were most bacteria Isolated from drink water.

Curing of Plasmid DNA Content of Bacteria Isolated from Urinary Tract Infection

Mohammed Z. Said; Khalid D. Ahmed

Rafidain Journal of Science, Volume 21, Issue 1, Pages 16-25

Twenty-five bacterial isolates were collected from patients with urinary tract infection from Hospitals in Mosul city. The isolates were identified by the morphological, cultural and biochemical tests . The results showed that 18 isolates belong to Escherichia coli, 3 Klebsiella pneumoniae, 3 Proteus mirabilis and 1 Pseudomonas aeruginosa. The resistance of these bacterial isolates was tested against antibiotics and heavy metals such as mercuric chloride, cadmium chloride and nickel chloride. Four isolates belonging to different genera; E. coli, K. pneumoniae, Pr. mirabilis and P. aeruginosa chosen to eliminate their plasmid DNA content by using different curing agents; the antimicrobial agents Erythromycin, Nalidixic acid, Methoprim and Chloramphenicol were used at one half and one quarter of the minimum inhibitory concentration(MIC). Nalidixic acid was the most effective one in curing antibiotic and heavy metal resistance in K. pneumoniae in a frequency ranged between (4-100). The acidic pH at a value of 5.5 was used to cure the bacterial isolates resistance and P. aeruginosa was the most effected one and it showed a ratio of (11-96%) while E. coli revealed curing of studied traits by elevated temperature 44 °C in a ratio of( 4-100%) . The effect of these curing agents on some other phenotypic traits was studied and mucus production of K. pneumoniae cured at a frequency of 40% by Nalidixic acid.

Histopathological Study of Placenta in Cases of Hypertension During Pregnancy

Iwatif A. Al-Taee; Hamad J. Jumaa

Rafidain Journal of Science, Volume 21, Issue 1, Pages 26-39

This study was carried out in order to investigate the histopathological changes in the placenta of hypertensive pregnant women. A collection of forty placenta samples from hypertensive pregnant women associated with forty placenta samples from normal pregnancy selected as control group. The pregnant women were admitted to Al-Khansaa Maternity hospital in Mosul.
The histopathological changes in hypertensive placenta included fibrosis with stenos is of maternal arteries, shedding and discontinuation of syncytial border in the decidua, hemorrhage inflammatory exudates and necrosis in the decidua basalis. Accumulation of huge amount of fibrinoid deposits was present in maternal decidua and large amount of collagen fibers surrounded the villous stroma and maternal decidua.
A possible explanation for the results of this study is that the hypertension in pregnancy have enormous affect on inducing histopathological changes in the placenta of hypertensive pregnant women

Application of Low pH as a Curing Agent of Plasmid DNA in Streptomyces as Compared with other Agents

Rayan M. Faisal

Rafidain Journal of Science, Volume 21, Issue 1, Pages 40-53

Fourteen strain of Streptomyces were obtained from a previous study and their identity was ascertained by using slide culture method that showed their aerial and earthy hyphae. Antibiotic susceptibility of the strains towards Nalidixic acid, Ampicillin, Chloramphenicol, Cefalexin, Rifamycin and Gentamycin were tested and the four most resistant were selected for subsequent analysis. Spontaneous curing was obtained as a control sample and we noticed (0-4%) curing percentage, after treatment of the isolates with acidic pH we noticed (22-100%) curing percentages towards the antibiotics understudy .Then we used various chemical and physical curing agents to compare them with acidic pH treatment. We found that elevated temperature, acridine orange (50g/ml), (100g/ml), SDS (50g/ml) and (100g/ml) had the ability to cure the isolates at various percentages (4-60%), (0-20%), (0-12%), (0-6%), (4-20%) respectively. This indicated that using the acidic pH has an effective curing ability to cure the plasmid DNA of Streptomyces much better than other well known curing agents .Low pH is also much easier and safer to handle as compared with chemical compounds that can be mutagenic to chromosomal DNA and harmful to the researcher himself .

A qualitative and A quantitative Study of Free Pool Amino Acids in Redia and Cercariae , (Furcocercous) which Infect Lymnaea aurcularia Snials

Muhammed S. A. F. Al-Salihi

Rafidain Journal of Science, Volume 21, Issue 1, Pages 54-61

Thin layer Chromatography technique was used to determine the free pool amino acids in rediae and cercariae (Furcocercous) wich were obtained from Lymnaea aurcularia snials, collected from Tigris river (Al-Rashedeya area) in Mosul city. The qualitative analysis suggested presence of free amino acids : alanine, leucine, lysine, histidine and valine in the redia, while: alanine and histidine were found in the cercariae, by using nihydrine reagent as to the quantitative determination for these free amino acids they were 0.002-0.005 μg/ml in both redia and cercarie.

Diagnosis of Autoimmune Hepatitis By ELISA and Indirect IF

Mahmood A. Husain; Mahmood M. Nasser; Mohmad M. Abiad

Rafidain Journal of Science, Volume 21, Issue 1, Pages 62-62

The diagnosis of Autoimmune hepatitis type-1 was studied in 167 blood samples which collected from Aleppo university and Zahi Azrak Hospitals in Aleppo, patients were complain in symptoms of hepatitis during the period from 4 – 2008 to 4 - 2009 . By determining special auto antibodies existence and in the comparison technique will be used the (ELISA), and the (IFA) technique. The results did not showed any infection with autoimmune hepatitis type-2, while the disease percentage in the type-1(AIH) 27.0%, the ANA was 13.33%, ASMA percentage 33.33%, and the AMA , 13.33%, The results did not show LKM-1 & SLA. (LKM-1 special in the Autoimmune hepatitis type-2) of the studied patients.

Using Monte Carlo Method for Calculating Phonon Images

Momtaz M. Hussien; a Z. Khalel; Asma

Rafidain Journal of Science, Volume 21, Issue 1, Pages 73-89

In this paper a Monte Carlo method has been used to calculate the phonon images of a number of semiconductor cubic crystals from III-V and II-VI groups (higher symmetry) and orthorhombic crystals (lower symmetry). An algorithms a MATLAB codes has been prepared for the calculation of phase velocities, slowness surfaces and group velocities and the formation of the phonon images which required a large number of points in the reduced Brillion zone, these points has been transformed to the group velocity space by solving the Christoffel equation for each of these points, a projection in a given direction is obtained, each incident pluses is transformed to gray level to form the final image. A comparison between the Monte Carlo and systematic methods has been done for the calculated images.

The Effect of the ِAcid HCl Concentration on the Optical Properties of P-Crystal Violet

Zahraa Z. Yousif; Ismail K. Abbas

Rafidain Journal of Science, Volume 21, Issue 1, Pages 90-100

Experimental study has been presented for calculation the optical properties of organic semiconductor crystal violet in the range(300-1000)nm .This material has high absorption in the visible range, so it can be used as solar cell. Also the study gave the result that this material is opaque in the visible range . The energy gap is (2.51eV) in the range (300-440) nm. The optical conductivity was increased by increasing the incident photon energy .It appeared that by adding HCl to the crystal violet, the band gap value was affected and it was (2.2 eV). The crystal violet has optical activity property with rotatory coefficient 5.2*10.