Volume 19, Issue 6, Autumn 2008, Page 1-192

Inhibitory Effect of Lowsonia inermis Leaves on Staphylococcus Aureus Isolated from Different Cutaneous Infections

bdul Razak K. Mahmood; Rwaa Hamoshi

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 1-19
DOI: 10.33899/rjs.2008.41395

The study included the isolation and identification of Staph. aureus from different cutaneous infections in the emergency unit, and out patients of dermatology clinic both within the Mosul Medical City. (247) samples were collected, (155) of them were of
Staph. aureus at a ratio of (62.7%). The highest rate Staphylococcal infection ( 76.1%) was isolated from impetigo while the lowest rate ( 37.5%) was isolated from infected wounds. The inhibitory effect alcoholic and aqueous, petroleum ether, benzene, chloroform, and acetone of Lowsonia Inermis leaves against the isolates of Staph. aureus. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) determined for these extracts.
The results indicated that the acetone extract showed the best effect on bacteria and the MIC was (0.5 mg/cm3) and the MBC equal to (1.0mg/ cm3) against the tested bacteria. The MIC and MBC differs according to the type extract and the region of isolation .
The Acetone extract was chosen to test its effect in vivo , this extract showed good results in treating the experimental wounds which were artificially infected with Staph. aureus using the Albino rabbit as experimental model for maximum period of twenty days .

Isolation and Identifcation of Micro–Organismٍٍs in Cervicitis and Vaginitis From Women in Mosul City

Basima A. Abdulla; Modrika M. Al-Jammaly

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 20-31
DOI: 10.33899/rjs.2008.41399

The study included isolation and identification of the causative micro-organisms of cervicitis, vaginitis.
A total of (202) samples (wet vaginal swabs, high cervical dried swabs) were collected from women aged between (16-<45) years suffering from vaginitis or Bacterial Vaginosis and having clinical symptoms and were diagnosed by the consultants of the general hospital in Mosul during the period September 2003 - May 2004.
The percentage of the presence of pus, red blood, epithelial, clue cells, Candida albicans and Trichomonas vaginalis were determined and was considered as the primary indicator for cervicitis and vaginitis.
The results also showed that Bacterial Vaginitis and Yeast Vaginitis were predominates of the population examined especially for the age group (16-40) years old suffering from abdominal pain and increased vaginal discharges.
The results showed that Candida albicans was dominant then Escherichia coli, Staph. aureus, Klebsiella pneumoinae, G. vaginalis, Strept. spp., Oligella spp. Isolated for the first time in Iraq.
Mobiluncus spp., Streptococcus faecalis, Staph. epiderimdies, Staph. saprophyticus, Proteus mirabilis, Actinomyces, Pseudomonas aeruginosa, Bacteriod fragils, Sarcina lutea, Enterobacter, Alcalgenous spp. and Neisseria gonorrhoea.
Antibiotic sensitivity of isolated microorganism were tested and the results indicated that Ciprofloxacin as the most effective while Ampicillin had no effect.

Estimation of Cd, Zn in Soil of Different Site of Mosul Area and Their Effect on the Growth, Metal and Chemical Content in Spinach

Farah S. Saleh

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 32-45
DOI: 10.33899/rjs.2008.41402

The present study was conducted, to estimate the cadmium and zinc metals in soil of different regions of Ninavah province (Al-Rashedia, Al-Shreghan, Al-Kuba, Al-Gofran) and the effect of accumulation and the consequence of its accumulation on plant physiology and growth on different plants structure in spinach plant.
The result showed a significant increase in cadmium and zinc concentration in soil and plant structure planted in al- Rashedia in comparison to other regions. That caused a significant decrease in plant growth measured indicated by a significant decline in plant shoot and root hight. The dry weight of shoot and root, chlorophyll a,b. relative water content, protein, carbohydrate in leaf tissue and nutrition elements K, Mg, Ca. Results also showed a significant increase in prolein of leaf tissues and Na, Cl in plant in comparison to other studied regions.

On the Possible Fusion and Haploidization of Nuclei Induced by Griseofulvin in Aspergillus amstelodami

Hiba K. Mahmood; Sahi J. Dhahi

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 46-56
DOI: 10.33899/rjs.2008.41403

The present work aimed to investigate two cytological aspects of the antifungal Griseofulvin (GRF) in the ascomycetous fungus Aspergillus amstelodami. The first was its ability to induce nuclear fusion and produce heterozygous diploids in the heterokaryotic mycelia, the second was its ability to induce haploidization of such diploids. To that end four sublethal or subinhibitory concentrations 500, 1000, 1250, 1500 g/ml were tested. Within the experimental conditions specified in the present work none of these two endpoints was fulfilled.

Numerical Classification by Cluster Analysis of Thermoactinomyces Species as Compared with Some Species of Two Genera Bacillus and Paenibacillus

Essra G. Al-Sammak

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 57-69
DOI: 10.33899/rjs.2008.41405

Isolation of species belong to endospore forming Thermoactinomyces were cared out from 27 soil samples and compared with thermoresistant bacilli belong to two genera Bacillus and Paenibacillus.
The number of Thermophilic Actinomycetes in primary isolation was (1-4)
colony /gm/102 but that of Bacilli was (3-137) colony /gm/102.
Morphological, biochemical, physiological characters and sensitivity to antibiotics were studied for (35) isolates, and classified numerically by using simple matching coefficient (Ssm) and average linkage method.
Two main clusters A and B were cared out from the herarichichal tree in
which cluster A included 3 sub clusters, A1 included 3 strains of the species Thermoactinomyces vulgaris, the sub clusterA2 included one strain of the species Thermoactinomyces intermedius, and A3 included 9 strains of the species Thermoactinomyces vulgaris, were the sub cluster B1 included 15 strain of the species Bacillus mycoides, and the sub cluster B2 included 7 strains of the species Paenibacillus spp.
This indicated the close relationship between the different species which belong to same group.

Genetic Analysis of Diallel Crosses in F2 Bread Wheat (Triticum aestivum L.)

Najeeb K. Yousif

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 70-86
DOI: 10.33899/rjs.2008.41408

Six parents (Pandas, Gemeny, Saberbey, 69-S3, 35-S6, and Kvz/Cgn) and their F2 hybrids obtained by the selfing of F1 ,S from half diallel wheat crosses were used to estimate general combining ability for their F2 hybrids, gene effect through variance components and graphical analysis for maturity time, plant height, grain yield ,number of spikes / plant, spike length, 100-grain weight and number of grains spike. Seeds of parental varieties and their f2 generations from diallel crosses were grown at the plant experimental station, College of Education, Mosul University, during the growing season (2003-2004) according to the Randomized complete Block design with four replications, under rainfall conditions. The results showed highly significant variances for both general and specific combining abilities for all studied characters, this indicates the presence of additive and non-additive gene effects for these characters. Narrow sense heritability values ranged from 0.15 for number of grains / spike to 0.73 for 100-grain weight. The average degree of dominance and the graphic analysis revealed the partial dominance for 100-grain weight and over dominance for the remaining characters.

Study of the S-Layer Protein in Legionella pneumophila and Listeria monocytogenes

Mayada A. Al-Taee; Ghada A. Al-Taee; Amera M. Al-Rawi

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 87-96
DOI: 10.33899/rjs.2008.41414

The presence of surface layer was detected in each of Legionella pneumphila (gram negative) and Listeria monocytogenes (gram positive) by extraction of S-layer proteins then separated by using sodium dodecyle sulphate poly acrylamide gel electrophorasis (SDS-PAGE). Five proteins bands appeared for both types of bacteria.
The freezing effect on the S-layer protein of L. monocytogenes was studied by exposure to low temperature at -20C for three months. Six protein bands were detected,, as well as their differences in approximate molecular weights for the freezing isolate in comparison with non-frozen isolate.

Effect of Different Amino Acids as Nitrogen Sources on Polysaccharide Production ( Pullulan ) By Aureobasidium pullulans ATCC 42023

Shimal y. Abdul- Hadi

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 97-103
DOI: 10.33899/rjs.2008.41417

The effect of different amino acids as nitrogen sources in a basal synthetic medium on the production of pullulan by Aureobasidium pullulans ATCC 42023 was investigated. The results showed that glycine was the best nitrogen source among anthor amino acids tested in stimulating pullulan production . Maximum production of pullulan (21.16) g/L was obtained when basal synthetic supplements with 0.05% glycin as a nitrogen source.

Isolation and Studying The Active Compounds from Ginger (Zingiber officinalis) in Normal and Diabetic Rabbits

Luay AL-Helaly; Mohamed B. AL-Saadon

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 104-119
DOI: 10.33899/rjs.2008.41421

The study was included isolation of cold proteinous and non-proteinous extracts of Zingiber officinals roots. The study also comprised the isolation and studying the proteinous compounds, using gel filtration technique which produced two compounds A (M.wt 108542 D) and B (M.wt 3247 D).
The proteinous and non-proteinous compounds were administrated interaperitio-neally. After one week from the treatment, the results were indicated that the proteinous precipitate and (B) proteinous compound at the doses (150,75) body weight which were caused a significant decrease (P < 0.05) in serum glucose (glu), total cholesterol (TC), total lipid, LDL-C levels , with an associated significant increase (P<0.05) in serum HDL-C level, Glutathion (GSH) and superoxide dismutase (SOD) enzyme activity in liver, kidney and heart tissues, in normal and alloxan-induced diabetic rabbits.
Finally, It was suggested the proteinous compound B has antidiabetic and antioxidative stress effect in normal and diabetic rabbibts.

Using a Device of Spectroscopic Ellipsometry for Studying the Effect of Changing of the Absorption Coefficient on the Optical Storage MR/BDK System of Different Concentration

Namir B. Mohammad; Taha M. AL-Maula

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 120-127
DOI: 10.33899/rjs.2008.41424

The effect of absorption coefficient on the optical storage system MR/BDK
have been studied, optical storage system was prepared from example of methyl red (MR) in different degrees of acidity and mixed with benzyl dimethyl ketal (BDK) as aphotoinitiator in acertain rates of size, the optical properties measured by using a variable angle spectroscopic ellipsometry instrument. And the relation between the optical characterestics of the system and the absorption coefficient studied as well it has become clear that the increase of basic concentrations of the system, this leads to decrease of the used wave lenglength, and this in turn leads to increase of storage ability of the data, from this we obtain the result that the disks which prepared by basic concentras lions are better than that prepared from acid concentrations, and add this information to the library of programming of spectrum device.
Keywords: optical storage system, absorptiom coefficient, benzyl dimethyl ketal (BDK), variable angle spectroscopic ellipsometry.

Preparation and Study Absorption Coatings from Two layers Ferrite (MnFe2O4) and Different Metal Oxides within Electromagnetic Frequency Bandwidth (8-12.5)GHz

Ammar Y. Al-Jubory

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 128-143
DOI: 10.33899/rjs.2008.41427

Two layeres coatings were prepared one of them is from different metal oxides (TiO2, Fe2O3, ZnO, MgO, CuO, CdO, Ni2O3, MnO2) and the other is from ferrite powder (MnFe2O4) for each sample. Absorption measurements for electromagnetic (EM) waves in the frequency range (8-12.5)GHz were achieved by the standing wave ratio SWR instrument. In addition a study of the effect in exchanging the oxide layer with the ferrite powder layer when they are radiated by (EM) waves. It was noticed that the oxide layer (MnO2) and the oxide layer (Ni2O3) were the best among other prepared samples as they posses nearly equal absorption values with the ferrite layer when they are subjected to the (EM) waves directly and in exchange with the ferrite layer. The (MnO2) oxide with the ferrite showed an absorption value of (87%) at the frequency range (10.2-11.4)GHz, while the (Ni2O3) oxide with the ferrite showed an absorption value of (80.5%) at the frequency range (10.8-12.4)GHz in comparison with other prepared samples.

Study of the Optical Properties PMMA/BDK/MR System Using the Spectroscopic Ellipsometry

Taha. M. AL-Maula

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 144-153
DOI: 10.33899/rjs.2008.41429

In this research the optical properties of PMMA/BDK/MR system have been studied. This system was prepared from the Azo-dye of methyl red (MR) in different degrees of acidity and doped with poly methyl methacylate (PMMA). Benzyl dimethyl ketal (BDK) was used as photoinitiartor at certain rates of size, and studying its optical characterstics by using the variable angle spectroscopic ellipsometry. The results show that increasing of basic concentrations of the system the ground energy increases compered with the increasing of acidic concentrations system this leads to decreased of the wave lengths than, and this in turn leads to increase of the storage ability than the data. From this we can conclude that the disks which prepared by basic concentrations are better those prepared at acidic than concentrations.

Study of Reference Evapotranspiration in Ninava Governorate Using Different Mathematical Models

Miklif Shalal; Kider Jasim; Waleed I.Al-Rijabo

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 154-173
DOI: 10.33899/rjs.2008.41432

تـم مقارنة نتـائج القـيم اليـومـية للتـبخر – نتح المـرجعي من أربعة نماذج ريـاضية
(Hargreaves and Samani, Modified Blaney–Criddle, Kharrufa, Pan) مـع نتائـج نموذج(FAO-56 Penman Monteith) لغرض ايجاد دقة كل نموذج وذلك في أربعة مواقع في محافظة نينوى. فـي محطة الموصل أظهر نموذج (Pan) دقـة أكبر من النماذج الأخرى حيث كان نسبة الخطأ المطلق لمعدل الانحراف MAE هو (%7.2). فـي ربيعة كان نموذج (Hargreaves and Samani) أفضل مـن النماذج الاخرى اذ كانت قيمة MAE (%7.5). في تلعفر كان نموذج (Modified Blaney–Criddle) أكثر دقـة مـن النماذج الاخرى بواقع (4.2) MAE%. أمـا فـي البعاج فأفضل النماذج كـان(Hargreaves and Samani) بواقع (%9.4). تـم ايجاد علاقات الارتباط بين نتائج هـذه الموديلات الأربعة مـع نتائج نموذج Penman Monteith. كما تـم إيجاد علاقات الارتباط للقيم اليومية لنموذج Penman Monteith مـع مختلف المتغيرات المناخية (التبخر، سرعة الرياح، درجات حرارة الهواء، الرطوبة النسبية، صافي الاشعاع، الاشعاع النسبي).

Theoretical Model for Semiconductor System GaP: Ni2+

Adnan M.AL-Sheikh; Heba M.T.AL-Hubaity

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 174-181
DOI: 10.33899/rjs.2008.41434

Studies of III-V semi-conducting compounds doped with Ni shows the need to introduce a theoretical model for GaP:Ni system.
A theoretical model using orthorhombic strain have been constructed with represent a promising approach to evaluate the energy levels for this system and interpret the experimental data of Zeeman studies of GaP:Ni2+ which have ground term 3F split with in tetrahedral crystal field into excited state 3T2 and ground state 3T1.

Study of the Effect Ratio Mixture for Active Material on the Frequency Response Curve for RAM Material

Faris B. Sofiya; Ammar Y. Al-Jubory

Rafidain Journal of Science, 2008, Volume 19, Issue 6, Pages 182-192
DOI: 10.33899/rjs.2008.41437

Frequency response curves in the microwave band (8-12.4)GHz of absorbing materials (RAM) involving (Ferrite–Iron) with Epoxy and Hardener mixtures are studied. The aim is to provide experimental data of the effect of the mixing ratio on both the frequency at which the maximum absorption takes place and the band width, the standing wave equipment SWR is used in this work, it is found that both maximum absorption frequency [f(max)] and band width tend to increase with increasing ferrite ratio and decrease iron ratio in the mixture.