Print ISSN: 1608-9391

Online ISSN: 2664-2786

Volume 19, Issue 3

Volume 19, Issue 3, Spring 2008, Page 1-55


Conjugal Transfer of Antibiotic Resistance Plasmid of Different Bacterial Species Isolated from Patients Suffering from Diarrhea

Khalid D. Ahmad

Rafidain Journal of Science, Volume 19, Issue 3, Pages 1-7
DOI: 10.33899/rjs.2008.41476

The transfer ability of antibiotic resistance plasmid DNA in the bacterial species Escherichia coli, Klebsiella oxytoca, Salmonella spp., Enterobacter cloacae and Morganella morganii which previously isolated from patients suffering from diarrhea, has been examined via conjugation. Plasmid DNA from the first four species carrying resistance for ampicilin, chloramphenicol, gentamycin, erythromycin and tetracycline and for heavy metals (mercury chloride, cadmium chloride and silver nitrate) was found capable of transfer to the laboratory E. coil strain (JM83) by conjugation which became resistant to these antibiotics and drugs The plasmid DNA transfer has been confirmed by analyzing the plasmid DNA content of the JM83 transconjugant colonies using agarose gel electrophoresis where the plasmid profile revealed the existence of the transferred DNA plasmid in the recipient JM83cells.

Effect of Aromatic Ester Groups on Electrical Conductivity of Polyazomethine

Asaad F. Khattab

Rafidain Journal of Science, Volume 19, Issue 3, Pages 8-15
DOI: 10.33899/rjs.2008.41480

Four types of copolymer have been prepared by solution condensation polymerization of azomethine monomer with four different types of acid chloride (phthaloyl, isophthaloyl, terephthaloyl and fumaryl).
The electrical conductivity of the copolymers have been enhanced by many order of magnititude according to polyazomethine or polyester. The ester group increased the electrical conductivity via two manners, increasing the flexibility of the chain, and making the charge carrier movement more easily along the chain.
The effect of temperature and doping with iodine on conductivity have been investigated. It was found that the electrical conductivity of these types of polymers is deffected by the distortion of the phenyl ring and the flexibility of the chain.

Indirect Spectrophotometric Method for Determination of Bromhexine-Hydrochloride in Pharmaceutical Preparations

Shilan A. Omer; Nabeel S. Othman

Rafidain Journal of Science, Volume 19, Issue 3, Pages 16-27
DOI: 10.33899/rjs.2008.41487

A sensitive indirect spectrophotometric method is developed for the determination of bromhexine-HCl in pure and dosage forms. The method is based on the oxidation of bromhexine-HCl with iron(III) in acidic medium, then reaction of iron(II) with 1,10-phenathroline to produce a ferroin complex which is water-soluble, stable, and has a maximum absorption at 510 nm against the reagent blank with a molar absorptivity of 3.51×104 l.mol-1.cm-1. Beer’s law is valid over the concentration range of 10 to 220g bromhexine-HCl in a final volume of 25 ml. The proposed method has been applied successfully to determine bromhexine-HCl in different pharmaceutical preparations.

Spectrophotometric Assay of Pyridoxine Hydrochloride (Vitamin B6) in Pharmaceutical Preparations and Serum Via Arsenazo III- Cerium (III) Reaction

Azzam A. Mosa; Raeed M. Qadir

Rafidain Journal of Science, Volume 19, Issue 3, Pages 28-41
DOI: 10.33899/rjs.2008.41495

A simple, rapid, accurate and precise spectrophotometric method is proposed for determination of pyridoxine hydrochloride (Vitamin B6) in pure form and in its pharmaceutical preparations and serum. The method is based on the oxidation-reduction reaction between vitamin B6 and cerium (IV) ion (ceric ion), then the subsequent reaction of cerium (III) with arsenazo III reagent in acidic medium in the presence of the neutral surfactant (Triton X-100) to produce a green complex which is stable, water soluble and has a maximum absorption at 716 nm with a molar absorptivity of 1.12105 l.mol-1.cm-1. Beer’s law is obeyed in the concentration range of 1 to 14 g of vitamin B6 in a final volume of 25 ml. The proposed method has been applied successfully to the assay of pyridoxine hydrochloride in pharmaceutical preparations and serum.

Synthesis and Characterization of Palladium (II) and Platinum(II) Complexes with Quinazoline and Thiolate Derivatives

Samir S. Ezzat

Rafidain Journal of Science, Volume 19, Issue 3, Pages 42-47
DOI: 10.33899/rjs.2008.41503

A number of Pd+2 and Pt+2 complexes with 2-methyl-3-aminoquinazoline 4-one (L1) and 2-methyl-3-hydroxyquinazoline-4-one (L2) have been synthesized by direct reaction of Na2PdCl4 or K2PtCl4 with the ligands. Complexes of the general formula [M(L)(SC6H4.p-Me)2] were also prepared through neucleophilic substitution reaction. The prepared complexes were characterized by IR and UV/Vis spectroscopy, conductivity, magnetic measurement and the metal content were determined spectrophotometrically. The electronic spectra and magnetic susceptibility indicates that the complexes have a square planer geometry in which the ligands behave as bidentate.

Inhibition of Partially Purified Xanthine Oxidase Activity from Sera of Patients with Gout

Mohammed B. Alsaadon; Omar Y. Al-Abbasy; Nashwan I. Al-Leheebi

Rafidain Journal of Science, Volume 19, Issue 3, Pages 48-55
DOI: 10.33899/rjs.2008.41513

In this research, xanthine oxidase (XO) was partially purified from sera of patients with gout, by dialysis and anion exchange chromatography techniques. One protein peak of XO activity was obtained with specific activity (0.149) unit/mg protein and with purification fold of (496.66) compared to crude enzyme.
Inhibition of partially purified XO was studied by allopurinol, caffeine and allopurinol with caffeine as a prodrug. The lower concentration of each inhibitor which exhibited maximal inhibitory effect was (10-7) mM. The inhibition type of XO by using these inhibitors at these concentrations competitive inhibition.
The results show that Km value without inhibitors was (40) mM. However, was Km values were (58.8), (100), and (125) mM with inhibitors respectively, and Vmax value remained stable (0.135) unit/ml. The inhibition constant Ki values were (0.68), (0.4) and (0.32) mM, respectively.
Accordingly, these results indicate that inhibitory effect of allopurinol with caffeine was better than caffeine, and caffeine was better than allopurinol. We suggest the intake of herbs and plants containing caffeine alone and/or with allopurinol drug to prevent or treat gout.