Print ISSN: 1608-9391

Online ISSN: 2664-2786

Volume 17, Issue 8

Volume 17, Issue 8, Summer 2006, Page 1-70


Cyclic Voltammetric Determination of Coenzyme (Qo) Using Graphite Electrode

Sadallah T. Sulaiman; Haithem A.Alwahb; Sabah A. Hamodi; Amer Th.Al-Taee

Rafidain Journal of Science, Volume 17, Issue 8, Pages 1-7

The anodic oxidation and cathodic reduction of coenzyme Qo (CoQ0) were studied using cyclic voltammetry (CV) at graphite electrode (C) in Britton -Robinson buffer (B.R.B) of pH ( 2-9). The result shows that the charge transfer processes were reversible over all pH range. The method is used to determine CoQ0 in concentration range ( 8.7 ×10-5 – 1.9 × 10-6 ) M at pH = 2 with a reduction potential of – 0.325 V and – 0.235 V for reduction and oxidation processes versus Ag / AgCl sat. KCl reference electrode

Study of Adenosine Deaminase Activity in Serum and Erythrocytes from Patients Affected by Acute and Chronic Leukemia

Zahraa M. Hammodat

Rafidain Journal of Science, Volume 17, Issue 8, Pages 8-16

Adenosine deaminase specific activity was studied in serum and erythrocyte haemolysate for patients with leukemia.
The results show an increase with a significant difference of activity enzyme in serum and erythrocyte for patients compared with normal individuals of the same age and sex. The results obtained showed adenosine deaminase specific activity by unite of nmole of adenosine deaminated/min/mg protein in serum and erythrocyte haemolysate of patients were (35.42±5.64) and (94.48±5.99) respectively, while the mean value of activity in normal individuals were (13.68±1.72) and (60.56±6.55) in serum and erythrocyte haemolysate respectively.
The analysis also indicated that factors of sex, age and type of disease Acute Lymphocytic Leukemia or Chronic Myelocytic Leukemia have no effect on the activity of enzyme in patient’s serum and erythrocytic haemolysate. Moreover, higher concentration of total protein the decline in calcium level is found in the serum of the patients, while no significant deference of alkaline phosphates and hemoglobin in leukemia and normal individuals
Finally, the statistical results has been shown that positive correlation between activity enzyme and total protein (r=0.369;p<0.05) and negatively with calcium (r=0.505; p=0.0001) in patient’s serum.

Parathyroid Hormone and Some Biochemical Parameters Level in Sera of Renal Failure Patients Undergoing Hemodialysis

Alaa M. Al-Layla; Talal S. Al-Najafi

Rafidain Journal of Science, Volume 17, Issue 8, Pages 17-28

This work involves estimation of parathyroid hormone (PTH) level in renal failure patients undergoing hemodialysis in Ninavah governorate. Sixty four samples collected from those patients were (35 male and 29 female) of age (15-66 years), were compared with fourty five samples as control group (22 male and 23 female) of age (15-63 years).
The studied biochemical parameters of blood sera, included, PTH, total calcium (T.Ca), inorganic phosphorous (PO43-), albumin (Alb) and alkaline phosphatase activity (Alp). The results showed high significant levels of PTH, PO43- and Alp. In the same time the study demonstrated that lower significant levels of T.Ca and Alb compared with control group (p < 0.05). The study of effect of the independent variables with respect to patients group on the level of the measured biochemical parameters were indicated that sex and number of hemodialysis per week had a significant effect on the level of PTH, while the period of treatment showed a significant effect on Alp levels. In the same time the age showed a significant effect on Alb level (p < 0.05).

Biochemical Study of Leptin and Insulin Hormones and their Relationship to Concentrations of Some Minerals in Diabetic Patients

Nawal Th. Younis; Kusia A. Al-chalabi; Thikra A. Al-Allwsh

Rafidain Journal of Science, Volume 17, Issue 8, Pages 29-40

The research, involved Biochemical study of leptin, Insulin and their relationship to some minerals in diabetic patients (Type I and Type II ) compared with normals .
The results demonstrated that the normal mean of Leptin hormone in human blood serum (19.5  5.6 ng / ml), and body weight affected Leptin concentration, while the results demonstrated a significant decrease in the level of Leptin and Insulin levels in serum of type I diabetic Patients in comparison with control and a significant difference in the mean levels of Leptin and Insulin between type I and II .
The results also demonstrated a significant decrease in the means of Potassium and Magnesium concentration in patients type I compared with control group, while non significant decrease of Sodium and Zinc in diabetic Patients type I and II compared with control .
Correlation coefficients between Leptin, Insulin hormone and some minerals of diabetic patients showed that Leptin hormone has a positive linear relationship with Magnesium and Zinc in control and diabetic patients type I and II, while Insulin hormone has a positive linear relationship with Magnesium and Zinc in control and diabetic patients type II, and showed a positive linear relationship with Magnesium in diabetic patients type I.
Also the results demonstrated a positive linear relationship between Leptin, Insulin hormone and Potassium in control group .

Study the Levels of Some Antioxidants and Biochemical Parameters in Patients with Duodenal Ulcer in Ninavah Provinance

Nabel N. Fathil; Azzam A. Musa; Tareq Y. Ahmad

Rafidain Journal of Science, Volume 17, Issue 8, Pages 41-55

The research included a biochemical study of patients with duodenal ulcer (DU). Histological biopsies were taken for the pathological and bacteriological tests, investigating H pylori infection. Blood (76 samples) and gastric juice (61 samples) were taken from patients with DU. The measured parameters in sera included :Vitamin C, Vitamin E, Vitamin A, Pepsinogen, total protein, albumin, chloride, iron, hemoglobin and blood groups, whereas in gastric juice included: vitamin C, Pepsinogen, chloride, total protein and urea.
The results showed a significant decrease in vitamin C, vitamin E, vitamin A, iron and hemoglobin concentration in the serum of patients with DU, in comparison with control group, for both sexes. Pepsinogen was also found to be increased significantly in both blood and gastric juice of patients with DU, for both sexes. A significant increase in blood and gastric juice chloride concentration was found for patients with DU for both sexes. The results had also shown no significant difference in blood total protein concentration for patients with DU whereas a significant decrease in gastric juice protein concentration was noticed for patients with DU for both sexes. It had pointed that serum albumin concentration was within the normal range for patients with DU for both sexes. Finally the results had demonstrated a significant decrease in gastric juice urea and vitamin C concentration for patients with DU in comparison with control group for both sexes. However, the results had also confirmed that persons with blood group O+ are more liable to have duodenal ulcer .

Isolation and Biochemical Study of the Active Proteinous Compounds from Coriandrum sativum Linn. Fruits

Manar M. Mahmood; Tareq Y. Ahmad

Rafidain Journal of Science, Volume 17, Issue 8, Pages 56-70

The research was an attempt to isolate and study the active protienous compounds from the cold aqueous extract of Coriandrum sativum L. using different biochemical techniques.Two compounds I and II were isolated using gel filtration chromatography of the precipitate produced by cold acetone precipitation.The comparative molecular weights of I and II were found to be in the range of 70800 and 4000 dalton respectively.
The work also included the study of the aqueous extract and the proteinous compounds on certain blood constituents and liver glycogen in normal and alloxan-induced diabetic mice. In normal mice, blood glucose level was lowered by concentrated aqueous extract and low molecular weight proteinous compound II to 54.4% and 50.4%. The aqueous extract and compound II also reduced the level of total cholesterol to 27.8% and 30.8%, whereas only the aqueous extract produced an increase in glycogen content in the liver of normal mice. In diabetic mice, the aqueous extract and compounds I and II lowered glucose level to 65.8%, 74.3% and 49.3% and also reduced the levels of total cholesterol and total lipids significantly. In addition, compound I showed a significant increase in glycogen content in liver of diabetic mice