Print ISSN: 1608-9391

Online ISSN: 2664-2786

Volume 16, Issue 13

Volume 16, Issue 13, Autumn 2005, Page 1-22


Glycogen Changes in the Rat Uterus During Different Phases of the Oestrous Cycle

Hafidh A. Al Ashou; Elias I. Shaya; Manhal S. Al Annaz

Rafidain Journal of Science, Volume 16, Issue 13, Pages 1-7

Objective: to study the glycogen content in the rat uterus at oestrous cycle (oes).
Design: an experimental study of the glycogen content in the rat uterus at (oes).
Setting: done during the period of 2000-2002 in the department of anatomy Mosul Medical College.
Methods: this study was carried out on 24 sexually mature virgin female rats of 13-15
weeks old and 165-270 gram weight. They were divided into four groups according to the phases in oestrus cycle 1. Metoestrus 2.dioestrus 3. Proestrus and 4. Oestrus phases, the uterine horns were divided into pieces of 3-4mm and prepared for histological examination, then sections of six micron thickness were collected and stained by alcian blue-periodic acid Schiffs’ reagent (PAS). The glycogen was shown as magenta color.
Result: cellular proliferation was noticed at dioestrus and proestrus phases then followed by an increase in the glycogen content at proestrus which became highest at oestrus phase, which is mainly concentrated in the endometrium.
Conclusion: this study showed increase in glycogen content at proestrus and oestrus phases mainly concentrated at the endomatrium, due to consumption of glycogen by the future nidating zygot after mating and fertilization which occur in the endometrium later

Worm Burden, Dispersion and Egg Count of Echinococcus granulosus in Stray Dogs of Mosul City, Iraq

an T. Jarjees; Ibraheem A. Abdullah

Rafidain Journal of Science, Volume 16, Issue 13, Pages 8-13

Worm burden, dispersion and egg count of Echinococcus granulosus were studied in Mosul city during 1997 to 1999. One hundred and twenty adult stray dogs were killed and necropsied for detection and determination of the actual number and dispersion of E. granulosus along the small intestine. Results revealed that worm loads ranged between 52 to 2000 worms with an average 1026 worm per dog. The commonest site of worms was the second third of the small intestine (75%), followed by the first third (20%). Few helminths were recovered in the last third which constituted 5% . The number of ova observed in the uteri of a gravid segment was 80 to more than 3000. The findings showed that 16.66% of the dogs harbour the cestode.

Relation Of Infection With Helicobacter Pylori and Vacuoles Formation Inside The Infected Cells.

Saba A. Sultan; Soubhi H. Khalaf

Rafidain Journal of Science, Volume 16, Issue 13, Pages 14-22

One hundred fifty patients who were included in this study visited the endoscopy unit these complaining from epigastric pain, dyspepsia, acidity, vomiting, abdominal pain, flatulance, heart bum and melena, All underwent esophagogastroduodenoscopy (OGD) and biopsies were taken, on which bacteriological and histopathological tests were done, aiming, first, isolation of Helicobacter pylori and second, looking for documents regarding the role of this bacteria in pathogenesis of peptic ulcer. Direct smear of biopsies were examined and then cultured on selected media of H.pylori. Histopathological examination was also done, using paraffin and plastic sections. When detection of H. pylori was considered, the highest sensitivity rate was found 74.1% when it was cultured on [Brucella agar + (5-7% )sheep blood + skirrow's supplement] while direct smear examination from biopsies was the second and it was 70%, the third position was obtained by histopathological examination of paraffin section and it was 67.9 %, on the contrary plastic section cames in the fourth place 43.5 %. Vacuole formation (in-vivo) was documented on histopathological examination and it was the most important finding as we think that may explain the role of H. pylori in peptic disease. The vacuoles then enlarge leading to cell ruptures and cell death knowing this fact we think peptic ulcer is caused by H. pylori.