Abstract
The field survey of broad bean fields in Nineveh showed the existence of BYMV in (Rashidiah, Guabba, Shrekhan, Shalallat and Danadan) regions in 2008-2009 and 2009-2010 seasons .
The viral incidence was 52% in Rashidiah at pre-flowering stage, while the incidence was 30% at Shalallat, whereas in 2007 season, the disease incidence reached 55% in Rashidiah and 36% in Danadan fields.
The results of serological diagnosis, using TBIA, Dot-Blot techniques proved that the main cause of mosaic symptoms on broad bean plants in these fields was BYMV (Bean Yellow Mosaic virus).
TBIA technique showed the existence of BYMV at 4% in broad bean seeds.
Spraying mineral oil (Sunoco) and insecticide (Alpha Methrin) separately and their mixture using corn plants as barrier plants revealed that the contents of potasium, calcium and sodium were decreased, but nitrogen content was increased in diseased plants 0.310, 0.343, 0.232 and 0.067 mg, respectively, but these values in case of using barrier plants were 0.532, 0.543, 0.596 and 0.042 mg, respectively.
Also treatments reduced the content of chlorophyll A and B in diseased plants to reach 17.94, 15.71 and 14.53 mg/g and increased in case of botanical barrier treatment to reach 32.61, 28.52 and 24.43 mg/g.