Micropropagation of Black Poplar Trees ( Populus nigra L. ) by Tissue Culture
Rafidain Journal of Science,
2013, Volume 24, Issue 12, Pages 1-17
AbstractA successful protocol was used for black poplar (Populus nigra L.) micropropagation through tissue culture, through initiation stage which studied, the effect of concentrations 0.0, 1.0, 1.5, 2.0, 2.5, 3.0 mg / l of Benzyl Adenin (BA) and explants of two different types (single node and shoot tips). In multiplication stage the effect of BA concentrations 0.0, 0.5, 1.0, 2.0, 3.0 mg / l alone or in combination with IBA or NAA 0.1. 0.3 mg / l. The results of initiation stage after four weeks of culture showed significant differences for single node application in comparison with shoot tips, by ratios value of response (80%, 68.33%) at a time the growing node has a significant superiority for growth in MS without regulators in relation with that MS medium having different concentrations of BA. But the results of the multiplication stage, after eight weeks of culture showed different cultured nodes of response to different concentrations of BA, the medium with 1.0 mg / l BA is significantly different for a number of shoot traits (2.7 shoot / node) and the longest shoots more than 0.5 cm. (2 shoot / node) in comparison with other concentrations studied of BA. On the other hand, the combination of 1.0 mg / l BA with 0.3 mg / l of IBA or NAA causes a significant increase for some studied characteristics in comparison with the control (1 mg / l BA) and other studied interactions by which we have got the highest number of shoots (4.7 shoot / node, 4.9 shoot / node) respectively in relation with control (2.6 shoot / node), also we have highest number of leaves (17.4 leaf / node, 17.2 leaf / node) and shoots longer than 0.5 cm. trait of (3.7 shoot / node, 3.5 shoot / node) respectively. The shoot regenerated from tissue culture rooted by ratios of 73.5 % in MS medium with half of the strength of salt with 0.5 mg / l IBA and then adapted and transferred to field conditions at success rate 90% .
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