Comparative Study for DNA Characteristic of some Parasitic Nematodes in Fishes and Frogs
Rafidain Journal of Science,
2013, Volume 24, Issue 8, Pages 47-63
AbstractThe present study has investigated the nematodes settled in fishes and frogs after being anatomized with numbers reached, 1246 of Silurus glanis, 1142 of Mastacembelus simach fishes, 490 of Bufo viridis frog. Five species of nematodes which are the Larva 4 of Eustrongylides sp., Larva 3 and 4 of Contracaecum sp. in fishes and mature nematodes Rhadias bufonis, Kathalania ninivana, Oswaldocruzia in frogs.
In this study, the DNA was isolated and its purity was tested as well as studying the effect of heat on the DNA by measuring its thermal denaturation temperature (Tm), and determining its G-C%. It was found that the isolated DNA has an evident absorption –peak at a wavelength of 260 nm. It was also found that the thermal denaturation temperature (Tm) of the DNA in different species of nematodes is convergent and has reached (84, 83, 83, 82, 81, 80) C° in larva 4 of Eustrongylides sp., and larva 3 of Contracaecum sp., larva 4 of the same nematode and the mature nematodes O. ninivana, K. ninivana and R. bufonis respectively. While it has reached G-C% (35.87%, 33.43%, 33.43%, 30.99%, 28.55%, 26.11%) for the above mentioned nematodes, respectively.
The study aimed to study some characteristics of DNA in different parasitic nematodes in fishes and frogs.
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