Mutagenicity of the Insecticides Vapcomic and Match in Conidia of Aspergillus amstelodami
Rafidain Journal of Science,
2013, Volume 24, Issue 6, Pages 16-27
AbstractThe ability of the insecticides Vapcomic and Match to induce genic mutation in conidia of the ascomycetous fungus Aspergillus amstelodami was tested. To this end ,the inhibitory effect on the radial growth of the fungus, of five concentrations (5.4, 7.0, 9.0, 18.0 and 27.0 (µg/ml)) of Vapcomic and (10, 20, 30, 40 and 50(µg/ml) ) of Match were tested. Three sublethal concentrations (giving inhibition of 50% or less of radial growth of the treated compared to the untreated colonies) of both insecticides were selected for mutagenicity tests. These were 5.4, 7.0 and 9.0 µg/ml for Vapcomic and 10, 30, 50 µg/ml for Match. The mutagenic action of each concentration of the insecticide was sought in the increase of the frequency of mutants resistant to the base analogue 8-azaadenine. Tests were made by two methods, plate incorporation and pretreatment. None of the treatments differed significantly from the spontaneous mutant frequency (which did not exceed 1×10-6 ) indicating the inability of the two insecticides to induce mutations according to the protocols adopted in the present work.
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