Indirect Spectrophotometric Assay of Hydroxyurea with its Pharmaceutical Application as Capsules
Rafidain Journal of Science,
2013, Volume 24, Issue 3, Pages 53-64
AbstractThe oxidation–reduction reaction between hydroxyurea and ferric ions has been utilised for spectrophotometric assay of hydroxyurea. The ferrous ions that are produced from the redox reaction were complexed with 2,2´-bipyridyl reagent to form a pink-red tris-chelate which has an absorption maxima at 522 nm. Beer's law agreed with the range of 5-150 μg of hydroxyurea per 20 ml, i.e., 0.25-7.5 ppm, with a corresponding average molar absorptivity of 1.65x104 l.mol-1. cm-1, Sandell sensitivity index is 0.0044 μg.cm-2, limit of detection 0.01μg.ml-1 and limit of quantitation of 0.03 μg.ml-1. The present method has been applied successfully to the determination of hydroxyurea in its pharmaceutical preparation as capsule.
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