Isolating and Studying Effect of Tannins, Oil, Flavonoids , Proteinous and non -proteinous Compounds from (Agrimonia Eupatoria) on Mice Exposed to Oxidative Stress
Rafidain Journal of Science,
2013, Volume 24, Issue 4, Pages 65-80
AbstractThe research is concerned with preparing a cold aqueous extracts of Agrimonia Eupatoria, to isolate oil, tannins, and falvonoids and proteinous and non proteinous compounds by gel filtration technique and using column containing gel type Sephadex G-50.Two compounds: A M.wt (264667) Dalton, B M.wt (3958) Dalton from the cold protienous precipitate were isolated. The effect of these extracts and the compounds mentioned above was studied on serum glucose, total cholesterol, triglyceride, high density lipoprotein-cholesterol (HDL-C), low density lipoprotein- cholesterol (LDL-C) and very low density lipoprotein-cholesterol levels, also glutathione (GSH) and malondialdehyde (MDA) levels in liver, kidney and heart tissues in mice exposed to oxidative stress via the intra-peritoneally injection.
The results obtained from this study showed that the cold crude aqueous, non-protienous extracts, protenious precipitate, proteinous and peptidic compounds, also the tannins, oil, and falvonoids at the doses of (300, 17.3, 3.04, 5.9, 10.3, 4.6, 5.28, 17.8) mg/kg body weights caused a significant decrease (p<0.05) in serum glucose, TC., TG., (LDL-C), (VLDL) and MDA level in liver, kidney and heart tissues. However, a significant increase (p<0.05) in serum HDL-C level and GSH level in liver, kidney and heart tissues in mice exposed to oxidative stress was observed while B peptidic compound caused a significant decrease in serum HDL-C level.
Finally, it was found that the falvonoids is more effective as a hypoglycemic and reactive oxygen spices
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