Behavior of some N-Substituted Phthalimides Derivatives as Plant Growth Regulators in the Initiation and Regeneration of Lettuce Lactuca sativa L. Callus
Rafidain Journal of Science,
2012, Volume 23, Issue 4, Pages 92-110
AbstractThe effect of twoi synthet phthailmides derivative compounds on the response of Lettuce seedling stem explants with single node was studied by addition to MS medium alone or with 3×10-6 M Naphthalene acetic acid (NAA) or with 4×10-6 M Benzyl adenine (BA).
The results indicated that the addition of 10-4 M of N-2(2-hydroxy phenyl) phthalimide compound (A) N-2-(2-carboxy phenyl) phthalimide compound (B) alone to MS solid medium increased the regeneration of shoots from seedling stem segments containing single node. However, addition of 10-8M of both compounds enhanced root formation. results showed that callus induced when 10-6 M of compound A added to MS medium .
Addition of 10-4 M and 10-6 M of both compounds A and B separately with the presence of 3×10-6 M NAA encouraged callus formation as in the standard medium, callus fresh weight was 23.30g and 23.06 g in the medium contains 10-8M of compound A or B with 4×10-6M BA respectively compared with 18.73g for the callus grown on standard medium after 60 days of growth. Whereas the fresh weight of callus grown on MS medium with addition of compound A or B alone reached 21.45g and 18.86 g respectively. addition of compound A and B separately with 4×10-6 M of BA enhanced shoot differentiation without formation of roots and from all the concentrations used 10-4 M of compound A was the best.
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