Isolation and Studying the Activity and Properties of Adenosine Deaminase (ADA) in Patients Tissues of Benign and Malignant Colon Tumors
Rafidain Journal of Science,
2012, Volume 23, Issue 3, Pages 117-130
AbstractThe present research includes determination of adenosine deaminase (ADA) activity in benign and malignant colon tumor tissues.
Patients were enrolled in the study to the surgery unit in AL-Jamhoory teaching hospital in Nineveh Governorate, samples of (6) patients (3) benign and (3) malignant colon tumors.
The results showed a significant increase in the activity of (ADA) in malignant tissues compared with benign which was (32.4 and 84.55 mU) in benign and malignant tumors respectively. Which represent about (50%) increase.
The study also included an isolation and partial purification of ADA using gel filtration chromatography from benign and malignant colon tumor tissues. the results indicated that the number of purification fold for the ADA was (10 and 12) from benign and malignant colon tumors respectively.
The approximate molecular weight of ADA by gel filtration using sephadex G-100 were (31622 Dalton, 39810 Dalton) for benign and malignant tumors respectively.
The optimum condition of ADA for the peak separated from malignant tumors showed an optimum reaction incubation time at (30) minutes, pH at (6.3), temperature at (37°C) and the substrate concentration was about (0.021mM). when we used linweaver - Burk plot, the maximum velocity (V max) and Michaelis -menten constant(Km) where found to be (31.25 mU/ml) and (10.310-3mM) respectively. Also in this investigation we was studied the inhibiters of ADA by Zn+2, Ag+1, Ca+2, Mg+2, Hgcl2, Fe+2 benign but not by Fe+3.
- Article View: 172
- PDF Download: 105