This study include detecting the role of some standard , growth regulators and one of the recently synthesized triazole derivatives, a compound that is classified to be growth regulator in initiation and growth of callus from stem segments of radish seedlings and the establishment of shoots and roots.
Cytokinine benzyl adenine (BA) is used with Auxins 2,4 –Dichlorophenoxyacetic acid (2,4,-D) and Naphthaleneacetic acid (NAA).
BA, 2,4,-D, NAA were used at several concentrations either alone or with growth regulators to select the ideal one which enhances initiation of callus and its growth. As for Triazole compound, it had been added to the nutritional medium at the concentration of 6- 10 molar either alone or with NAA, 2,4,-D, BA.
The results showed that adding BA alone to the medium stimulated shoot formation, whereas addition of BA with NAA stimulated the formation of shoot and roots.
Adding NAA to the nutritional medium alone stimulated the establishment of roots from the cultured explants, whereas addition of 2,4,-D alone to MS medium stimulated the initiation of callus.
The results showed that adding triazole compound with a concentration of (6- 10) molar alone to MS medium did not stimulate callus initiation, while shoots formed by this treatment.