Relation Of Infection With Helicobacter Pylori and Vacuoles Formation Inside The Infected Cells.
Rafidain Journal of Science,
2005, Volume 16, Issue 13, Pages 14-22
AbstractOne hundred fifty patients who were included in this study visited the endoscopy unit these complaining from epigastric pain, dyspepsia, acidity, vomiting, abdominal pain, flatulance, heart bum and melena, All underwent esophagogastroduodenoscopy (OGD) and biopsies were taken, on which bacteriological and histopathological tests were done, aiming, first, isolation of Helicobacter pylori and second, looking for documents regarding the role of this bacteria in pathogenesis of peptic ulcer. Direct smear of biopsies were examined and then cultured on selected media of H.pylori. Histopathological examination was also done, using paraffin and plastic sections. When detection of H. pylori was considered, the highest sensitivity rate was found 74.1% when it was cultured on [Brucella agar + (5-7% )sheep blood + skirrow's supplement] while direct smear examination from biopsies was the second and it was 70%, the third position was obtained by histopathological examination of paraffin section and it was 67.9 %, on the contrary plastic section cames in the fourth place 43.5 %. Vacuole formation (in-vivo) was documented on histopathological examination and it was the most important finding as we think that may explain the role of H. pylori in peptic disease. The vacuoles then enlarge leading to cell ruptures and cell death knowing this fact we think peptic ulcer is caused by H. pylori.
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