Rafidain Journal of Science

Abstract

The aqueous Allium sativum crude extract was tested in vitro by using the two standard techniques, Zone–of-Inhibition (Kurbey and Bauer) and Turbidometric assays against some of the common Brucella melitensis clinical isolates in Mosul . It was found that the concentrated aqueous crude extract (1000 mg/ml D.W) of A. sativum had the most powerful inhibition activity against the dose 1 × 107 viable bacteria /ml compared with the rest concentrations of A.sativum used. These inhibitory activities of A.sativum crude extracts were compared with the standard antibiotic zones commonly used for the brucella bacteria therapy. It was found that these following concentrations 0.002, 0.03, 0.008, 0.07 and 0.7-6 mg/ml were the standards for the antibiotics Tetracycline, Ampicillin, Erythromycin, Streptomycin and Rifampicin respectively. The in vivo work is under estimation in our laboratory. However, such encouraging results suggest the alternative herbal therapy against Brucella species in future.