Influences of Insulin, n-Acetyl Cysteine, and Folic Acid on the Level of Sex Hormones in the Diabetic Pregnant Rats
Rafidain Journal of Science,
2009, Volume 20, Issue 6, Pages 10-17
AbstractThe preventive influences of folic acid, N-acetyl cysteine (NAC), and insulin were examined on the alterations of reproductive system related hormones in alloxan-induced diabetic pregnant rats. Healthy pregnant rats (192) were treated with single subcutaneous injection 100 mg/kg body weight alloxan.
The experimental rats were divided into five groups: group one represented negative control, group two was positive control. Group three was treated with folic acid 0.25mg/kg; group four, daily injected with insulin; and last group was treated with NAC 1%. Each of 17- β -Estradiol; progesterone; follicle stimulating hormone (FSH) and luteinizing hormone (LH) were assayed by ELISA.
Alloxan induced diabetic pregnant rats showed significant decrease in all sexual hormone parameters and in all three weeks of gestation. The hormone β-Estradiol none significantly increased in all treatment groups, while significant increasing in progesterone value was observed in all treated groups. Insulin, restore the elevation of FSH and LH hormones except for LH in second week. Treatment with folic acid 0.25 mg/kg was showed significant increase FSH and LH except FSH in third week and LH in second week. While NAC showed less protective effects through decreasing of only progesterone and estrogen levels.
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