Measurement of Glutamate-Oxalacetate Transaminase Activity in Human Blood Serum Using Differential Pulse Anodic Stripping Voltammetry
Rafidain Journal of Science,
2010, Volume 21, Issue 8, Pages 11-18
AbstractA new electrochemical method for measuring the activity of glutamate-oxalacetate transaminase (GOT) in human blood serum was developed based on the appearance of a voltammetric peak current of oxalacetate at -0.4 V vs. (Ag/AgCl, 3 mol/L KCl) as a reference electrode using phosphate buffer (pH 7.4) as a supporting electrolyte. It is successfully applied for measuring GOT activity in certain diseases such as: Liver cirrhosis and Myocardial infarction, as well as normal cases. The results of our proposed method were compared with those obtained from colorimetric method for 38 specimens of human blood serum. A good relationship between the two methods were obtained with a correlation coefficient (r = 0.9976).
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