This study was conducted to investigate the protective effect of Grape seeds oil on some fertility parameters in white rats (Rattus norvegicus). This study used (36) male rats age (2.5-3) month, weights ranged (210-270) g which divided into (6) groups (6) rats/group. The first group (Control group), the second group (Tamsulosin group), the third group (Grape Seeds Oil 0.2 ml/kg) body weight, the fourth group (Grape Seed Oil 0.4 ml/kg) B.W, fifth group (Grape Seeds Oil 0.2 ml/kg+ 0.4 mg/kg, Tamsulosin). The sixth group (Grape Seed Oil 0.4 ml/kg+ 0.4 mg/kg. Tamsulosin). All treatment were given doses every day for (7) weeks. Result showed a significant decreased (p> 0.05) in testosterone levels in Tamsulosin group after the fourth and seventh group weeks of treatment as a compare with control group. Where the testosterone levels showed a significant increase in groups of Grape Seeds Oil 0.2,0.4ml/kg B.W after the fourth and seventh weeks of treatment. The fifth and sixth groups showed a significant decreased (p> 0.05) in testosterone levels after the fourth week, where group-two showed a significant increase after the seventh week. The results revealed a significant decreased in the total sperms count and the density of sperms and the percentage of living sperm (p> 0.05) in Tamsulosin group, and increased the percentage of dead and abnormalities sperms of Tamsulosin groups as compare with control group.