Rafidain Journal of Science

Abstract

Microbacterium sp. AJ-Z was isolated from fenugreek (Trigonella foenum-graecum) root nodules on Yeast Extract Mannitol (YEM) medium. Colonies of the bacteria isolates grown were rod shape, whitish, translucent, sticky nature. The results showed responded of the fenugreek seedlings grown on Nitrogen free medium (NF) to inoculated with Microbacterium sp. AJ-Z isolate at 30 min. and successful to form nodules its roots in the rate of 70% after 7 days with rate number of nodes/seedling was 2.9 superiority on the other which grown on Murashige and Skoog (MS) medium and 0.7% agar. Nodules produced have a spherical shape, pinkish and indeterminate in growth and after a few days, its shape becomes elongated.