The current research work was carried out to verify the role of AgNO3
silver nitrate in the germination and growth of seedlings of Leucaena leucocephala
trees and to demonstrate the effects on seedlings growth indicators such as stem height, root length, number of vegetative branches and number of leaves per seedling, as well as the induction of callus from stem and leaf explant, In presence and absence of silver nitrate in MS culture media. As well as different concentrations of silver nitrate were used, the continuous growth of seedlings that were grown on MS media and supported by different concentrations of AgNO3
showed to a variation in the response in terms of stem high and root length of seedling. As for the branches that arose after the cotyledon leaves and the number of leaves for these branches, they also varied as a result of using different concentrations of nitrates. Variation of the incidence of callus with the type of plant growth regulators and their concentrations used in this research, The study also included an explanation of the role of growth regulators in creating callus from cutting the stem and leaves and comparing it with callus growing on the same nutrient media and supplied with concentrations of silver nitrate.