Initiation Callus Cultures from the Seedlings of Silybum Marianum and Estimate their Protein Content
Rafidain Journal of Science,
2021, Volume 30, Issue 1, Pages 21-37
AbstractThe present research investigates the best Method for sterilization the Silybum marianum Plant seeds for getting best seedlings and the roles of plant growth regulators the (BA) Benzyl adenine and (2,4-D) 2,4-Dichlorophenoxy acetic acid, as well the type of the explants of the seedlings to induce and grow callus. In addition, determined the callus viability and its protein content. The results showed the best treatment seeds is to soak them with water for (24) hours and then with Ethanol 70% for (3) minutes, immerse them with Sodium hypochlorite) NaOCl) 2.5% for (10) minutes, and with 0.1% Tween 20 for (10) minutes. The explants of seedlings (roots, leaves, shoots, nodes) have been cultured on solid MS medium contain different concentrations (0.1,0.5,1.0,1.0) mg/L of BA and (0.25,0.5,0.5,1.0) mg/ L of 2,4-D. The medium which supplemented with (1) mg/l of (BA) and (2,4-D) had succeeded to induction of calls growth, which produce (2.4,3.1,4.1) gm. fresh weight respectively. The results showed that roots and nodes have more response than others to initiate and induce callus. The viability of callus showed a noticeable increase in age (80) day to reach (87%) for roots, leaves, and nodes callus. The callus of roots showed best increase in viability which reaches (95%) in (120) day. The results explained variation in protein level in callus culture with different in callus source and age, it reaches the highest level in nodes callus, and in (80, 120) days was the highest level of protein where in roots callus.
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