Histopathological and Micromorphometrical Study for the Effect of Stanozolol on Liver of Male Rats
Rafidain Journal of Science,
Volume 28, Issue 4, Pages 50-60
AbstractThe study aimed to investigate the histopathological and micromorphometric measurements of the effects of stanozolol with different concentrations and periods on the liver in male rats. The study included 80 male white rats divided randomly into four groups of 20 rats per group. The first included (control group) which get water and feed only. The second group was treated with stanozolol 10 mg / kg. The third group was treated with stanozolol 10 mg / kg body weight with Vit. E 600 mg / kg body weight and the fourth group was treated with vitamin E 600 mg / kg body weight only. All groups were treated for 8 weeks. Euthanasia was performed at 4, 8 and 12 weeks (4 weeks after cessation of treatment) and liver samples were taken by eight samples and for all groups. The histological sections of the liver in the second group showed presence of histopathological changes represented by fatty degeneration of hepatic cells with the expansion and congestion of sinusoid. Eight weeks later, the intensity of histopathological changes were increased, the sections showed severe fatty change. Four weeks after the treatment cessation at the 12th week, pyknosis of the nuclei of the hepatic cells were observed. In the third group, the changes after four weeksrepre sented by diffuse fatty change and sinusoidal expansion. After eight weeks of treatment, sinusoidal expansion with hypertrophy of kupffer cells were observed.
The micromorphometric measurements of the second group on the sinusoidal diameters showed a significant increase at p≤ 0.05 for all periods when compared with control group. The third group showed a significant increase at all periods and at a significant level p≤ 0.05 compared with control group, while it showed a significant decrease in the diameter of sinusoid at all periods and at a significant level p≤ 0.05 compared with the rats of the second group. The fourth group showed a significant decrease at the eighth week and four weeks after cessation of treatment (week 12) at most periods. The results of the micromorphometric measurements of the second group showed a significant decrease in the diameter of hepatic cell nuclei at a significant level p ≤0.05 and for all periods when compared with control group. The third group showed an insignificant increase in hepatic cell nuclei diameter at all periods compared with the treated group of 10-mg / kg body weight.
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