Spectrophotometric Estimation of Thiamine in Tablet form Application to Content Uniformity Testing
Rafidain Journal of Science,
2019, Volume 28, Issue 2, Pages 146-151
AbstractA simple, rapid, accurate and sensitive spectrophotometric method for the determination of thiamine has been developed. The method is based on the direct reaction between thiamine and sodium hydroxide to produce a yellow colored product having maximum absorption at 335 nm. Beer's law was obeyed over the concentration range of 2-20µg/ml,with molar absorptivity of 1.58x104 l/mol.cm. The present method was considered to be simple because it does not need heating, hydrolysis and solvent extraction steps. The ingredients often formulated with thiamine, have been shown not to interfere, and is suitable for the routine determination of thiamine. The proposed method has been successfully applied for the determination of thiamine in pure form and in pharmaceutical preparations(tablets).
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