Molecular Identification of the Local Isolated Fungi Aureobasidium pullulans
Rafidain Journal of Science,
2018, Volume 27, Issue 4, Pages 115-128
AbstractTwenty fungal isolates of Aureobasidium were isolated from many healthy leaves of
different citrus trees. Fungal isolates understudy were identified by using morphological and
cultural characters. The results revealed that the isolates are belonged to the species A .pullulans.
Molecular identification of the isolated fungi were carried out using the specific-PCR reaction. The
results showed 17 bands of purified genomic DNA from local fungal isolates of similar size ranging
(500-600)bp. These bands have the same size to that obtained from the standard strain A. pullulans
NRRL 58560. On the other hand, it did not show any band of the DNA as a result of the Specific-
PCR reaction specialize of isolates (Ap15 and Ap17 and Ap18) in the agarose gel.
The sequence of the nitrogenous bases for the Specific PCR products were determined for
three chosen local isolates. The results were analyzed using DNA Blast / NCBI program revealed
that there is a similarity higher than 95% between these sequences and those of the standard
strains of A. pullulans already recorded in the Gene Bank. Additionally, the point mutations
whether they are substitution or addition or deletion were observed in some positions of the
nitrogenous bases after comparing the sequences of the tested local isolates and the standard.
These mutations may occur spontaneously.
This analysis results sent to GenBank using the website of the NCBI and introduced in
GenBank record as standard isolates and given a cod number in the GenBank as follows: Ap1.sqn
Ap1 KX964610, Ap7.sqn Ap7: KX964611, Ap14.sqn Ap14 KX964612 This confirms the validity
of the results obtained in this study and diagnosis of the validity of the local isolates under study..
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