Keywords : Fusarium

The Effect of Biological Control Agents on Fungi Isolated from some Citrus Trees in Mosul

Maha M. Taha Al-Nuaimy; Faten N. Mula Abed

Rafidain Journal of Science, 2021, Volume 30, Issue 1, Pages 68-77
DOI: 10.33899/rjs.2021.167687

In this study, the counter effect of two Biological Control Agents, namely Trichoderma harzianum fungi and Bcillus subtilis bacteria against the fungi-like organism Phytophthora infestans and the fungi; Fosarium solani and Rhizoctonia solani isolated from orange- trees’ bark and from lemon- trees’ roots. The results showed that the counter effect of T. harzianum (using double culture on petri dishes) reached 1.0 according to Bell scale, against all these pathogens. The counter effect of this antibiotic was also tested in vitro on branches taken from citrus trees and infected with Phytophthora infestans. The infection percentagesbefore treatment with T. harzianum were 75% and 58.3% for the branches isolated from lemon trees and orange trees respectively, whereas after treatment with the inhibition fungi, the percentages of infection were 25% and 16.6% for the branches isolated from lemon trees and orange trees respectively. The percentages of inhibition by Bacillus subtilis bacteria were 83%, 71% and 73% against Phytophthora infestans , Fusarium solani and Rhizoctonia solani respectively

First Record of Fusarium poae, Wheat Root Rot Fungus in the North of Iraq

Ali K. AL-Taee; Zardasht A. Taha

Rafidain Journal of Science, 2018, Volume 27, Issue 4, Pages 162-168
DOI: 10.33899/rjs.2018.159351

The Results of field survey conducted in Ninevah and Erbil governorates to assess the
distribution of root rot disease in wheat have shown variability in infection levels. In Ninevah; the
highest infestation of %27.5 was reported from Bashika, and lowest of %16.9 from Hamdaniyeh,
and in Erbil;the highest infestation of %25.8 was reported in Grdy Mawan and lowest of %21.5
was in Khalwan. Isolates from root rot infected wheat plants shown the appearance of Bipolaris
sorokiniana (Sacc.) Shomaker, Fusarium graminearium (Schwabe ), F. culmorum (Wm. G. Smith )
Sacc and and F.poae (Schwabe ) as first found and recorded on wheat in Iraq, the fungus B.
sorokiniana recorded the highest isolation rate of %54.1 in Tallafar and lowest rate of %37.1 in
Qapakian, followed by F.culmorum which recorded a range of isolation between % 22.1 and
%25.4. Identification of isolated fungi were confirmed by conducting Polymerase Chain Reaction
(PCR) molecular test using 2 specific primers FP82F and FP82R. The expected PCR product (220
bp) obtained from a specific molecular marker (FP82), confirmed that they belong to fungus F.
Poae. However, this is the first molecular study indicates clearly the presence of F. Poae in Iraq.