Keywords : Entamoeba gingivalis

Effect of some Materials and Drugs on Entamoeba gingivalis Culture

Khansaa A. Younes; Ghada A. Taqa; Najah S. Nayef

Rafidain Journal of Science, 2019, Volume 28, Issue 4, Pages 40-49
DOI: 10.33899/rjs.2019.163300

The aim of this study was to investigate the effects of some materials and drugs on Entamoeba gingivalis culture which was isolated from gums in gingivitis patients who attended to the periodental disease department at the college of Dentistry/ Mosul University, at the period from August to October 2018.
Five different concentrations of Flagyl, Ketamine, Bowsella and Apignein were prepared then added to the culture media of the parasite  at three periods (2, 4, 6) hours. as (0.0001, 0.0002, 0.0004, 0.0008, 0.0016) microgram/ml. Statistical analysis showed that there were significant difference between concentrations and time which showed that best concentrations were 0.0016 mg/ml for Flagyl, Ketamine, Apignein which inhibit parasite growth as (0.0, 4.73j, 9.53g) respectively also reduced parasite survival rate at (100%, 72.81%, 54.4%) respectively in the time period at (6 hours) while Bowsella at 0.0008 mg/ml inhibition of parasite growth to (5.47l) also reduced parasite survival rate to (72.04%) compared together concentrations and time periods (2, 4) hours which showed slight differences between them.

Effect of Titanium Dioxide Nanoparticles (Tio2NPs) on Entamoeba gingivalis in vitro

Khansaa A. Younes; Najah S. Nayef; Ghada A. Taqa

Rafidain Journal of Science, 2019, Volume 28, Issue 4, Pages 1-7
DOI: 10.33899/rjs.2019.163297

The  effect  of  Titanium  Dioxide Nanoparticals (Tio2NPs) on Entamoeba gingivalis in vitro     were   studied,which isolated  from  gums  in  gingivitis patients who  attended  the periodontal  disease    department  at  the  Faculty   Dentistry  of  Mosul University  for  the  period  from  1/4/2018    to   31/7/2018.
In  our  study  three  different  concentrations  of  (Tio2NPs)  were  prepared  as  the following order: (0.1, 0.01, 0.001) mg/ml and evaluated the effect of these different concentrations through (2,4,6) hours. Results  showed that time factor and the increasing of the  appositive effect on the parasite killing and  the highest concentration (0.1mg/ml) had a role in inhibition of  parasite  growth  and  reduced  parasite  survival rate to (3.60±0.953) compared to the other  concentrations. Also in this present study we found the concentration (0.1 mg/ml) was the highest  inhibition  of  parasite  (86.73%)  in  the  time  period  at  (6 hours)  compared  to  other concentrations and periods time  which    showed   slight   differences    between    them.