Keywords : Superoxide dismutase
Rafidain Journal of Science,
2012, Volume 23, Issue 4, Pages 55-69
The present study includes the investigation of the effect of lead on some enzymatic, non enzymatic antioxidants and lipid peroxidation in serum of male albino rats Rattus norvegicus aged 2.5-3 months. Fifty male rats were randomly divided into 5 groups (10 rats/ group).The first group: was given standard forage and distilled water (as a drinking water) and considered as control. The second group: was given standard forage and distilled water (as a drinking water) containing 20 mg lead /L. daily for a period of 15 days. Third group: was given standard forage and distilled water (as a drinking water) containing 40 mg lead/ L. daily for a period of 15 days. The fourth and fifth groups were treated with the same concentration of the second and third group separately daily for a period of 30 days.
The results showed that treatment with lead caused a significant decrease in the concentration of each of total protein, albumin, globulin, glutathione GSH and the superoxide dismutase SOD activity in serum of treated rats compared with the control group. The reduction of the above mentioned parameters was progressive with the time of treatment. The result also showed that treatment with lead caused a significant increase in uric acid and malondialdhyde MDA in serum compared with control group. The results also showed that the decrease and increase were more prominent in rats treated with 40 mg
lead/ L of drinking water. These results suggests that lead may induce oxidative stress in albino rats.
Comparative Effects of Alpha – methrin (Serin) and Actara (Cruzer) Insecticides on some Enzymatic and Non-enzymatic Antioxidants and Lipid Peroxides in Serum and Tissues of White Male Albino Rats
Rafidain Journal of Science,
2010, Volume 21, Issue 4, Pages 1-20
The present study included the recognition of alpha-methrin (Serin) and actara (Cruzer) insecticides medium lethal dose LD50, which was found to be 150 mg alpha-methrin/Kg body weight and 1875 mg actara / Kg body weight. By consideration of (LD50) values three doses of each insecticide were orally administered to male albino rats for 15 and 30 days period, to study their effects on the levels of some enzymatic, non enzymatic antioxidans and lipid peroxides in serum and tissues of albino rats.
The rats were divided into 7 groups; the control group which administered with distilled water, the first to third group were administered with 2.5, 5, 10 mg /Kg body weight alpha-methrin respectively, the fourth to sixth group were administered with 100, 150, 200 mg /Kg body weight actara respectively on comparison to control group with that treated with either alpha-methrin or actara. The results showed a significant decrease in non enzymatic antioxidant (uric acid concentration and glutathione) level and an increase in the activities of superoxide dismutase (enzymatic antioxidant) and a significant increase in malondialdehyed level in serum blood of rats treated with either alpha-methrin or actara. The results also showed a significant decrease in level of glutathione accompanied with significant increase in malondialdehyed level in tissues of (brain, heart, liver and kidney). The rats which administered with different concentrations of alpha-methrin insecticide, showed more effects than that groups administeared by actara. These results indicated that the alpha-methrin has more poisonous effects than actara insecticide .