Keywords : glutathione


Analysis of the Oxidative Stress Parameters in Testicular, Hepatic and Renal Tissues Homogenates of Albino Rats after Administration of Imatinib at Peripuberty

Hafidh Al-Ashoo; Luma I. Al-Allaf

Rafidain Journal of Science, 2018, Volume 27, Issue 4, Pages 128-135
DOI: 10.33899/rjs.2018.159342

Background: Targeted small molecule drugs have revolutionized treatment of chronic
myelogenous leukemia (CML) over the last two decades. The safety zone of imatinib, and
specifically its relevancy to organ toxicity, has been discussed in a controversial manner in current
years. There is a little data on the contribution of oxidative stress in imatinib induced organ
toxicities
Objectives: This work aims to analyze the possible role of oxidative stress in the occurrence of
testicular, hepatic and renal toxicities induced by imatinib (if present) by analyzing some
parameters including glutathione (GSH), malondialdehyde (MDA) levels in their homogenates.
Study setting and design: This experimental study was conducted on male Albino rats (purchased
from Animal Houses of both Mosul Medical College, and Veterinary College, University of Mosul,
Mosul, Northern Iraq).
Methods: This work included 40- 45 days aged rats (at peripuberty) (n=8) which administered a
single daily dose of 200mg/kg of imatinib mesylate for 30 days (100mg capsule, Glivec®, STI 571;
Novartis) bought from Ibn-Sena Teaching Hospital, Mosul, Northern Iraq, or bought from some
private pharmacies and were dissolved in distilled water (D.W) and given orally by gavage with 24
gage needle. Another group of 8 rats were administered with D.W represents an age matched
control. Testes, livers, parts and kidneys were collected from all rats. Tissue GSH, MDA
concentrations in the homogenates of the three organs were determined by spectrophotometer
according to the methods described.
Results: All rats survived the experimental period. This study revealed a decrease in value of GSH
in the testicular and hepatic tissues of the treated rats, however, the concentrations of this
antioxidant in renal tissue were higher than that of control ones. Briefly, imatinib induced an
elevation of MDA productions in both testicular and hepatic tissues when compared to the control
group. MDA concentrations in renal homogenates in the treated group were lower than that of
control ones.
Conclusion: Imatinib induced a decrease in the level of GSH and increased in level of MDA in
testicular, hepatic homogenates indicating its oxidative effect.الخلفیة: ان الأدویة المستهدفة للجزیئات الصغیرة قد احدثت ثورة فى علاج مرض ابیضاض الدم المزمن خلال العقدین
المنصرمین. ان المنطقة الامنة للایماتنب وخصوصا علاقته مع سمیة الاعضاء قد نوقشت بطریقة متناقضة فى السنوات
الحالیة. هناک بیانات قلیلة عن مساهمة الکرب التاکسدى فی سمیة الأعضاء الناتجة عن الایماتنبأهداف الدراسة: یهدف هذا العمل الى تحلیل الدور الاحتمالی للکرب التاکسدى فى حدوث سمیة الخصیة والکبد والکلى الناتجة
عن الایماتنب (اذا ما کانت موجودة) وذلک عن طریق تحلیل بعض المؤشرات التی تشمل مستویات الکلوتاثایون المالوندالدیهاید
فی مزیج أنسجة تلک الأعضاء.
مکان الدراسة: هذه الدراسة التجریبیة اجریت على الجرذان المهقاء والمهداة من بیتی الحیوانات التابعین لکلیتی طب الموصل
والطب البیطرى، جامعة الموصل فى مدینة الموصل شمالی العراق.
45 یوما (حول البلوغ) وعددهم ثمانیة وذلک عن طریق الفم یومیا - طرائق العمل: تضمن العمل تجریع الجرذان بعمر 40
بمقدار 200 ملغرام لکل کیلوغرام من وزن الجسم ولمدة شهر بعقار الایماتنب مسیلیت (کلیفک، نوفارتس) مهداة من مستشفى
ابن سینا التعلیمى، مدینة الموصل او مشترى من بعض الصیدلیات الخاصة. تم اذابة الدواء فى الماء المقطر وباستعمال ابرة
تجریع قیاس 24 . مجموعة اخرى من نفس العمر جرعوا بالماء المقطر ومثلوا مجموعة السیطرة. تم جمع اخذ الخصیتین
والکبد والکلیتین من کل الجرذان. تم تحدید تراکیز الکلوتاثایون والمالوندالدیهاید فی مزیج أنسجة الأعضاء الثلاثة بجهاز قیاس
الطیف الضوئی وذلک حسب الطرق المعتمدة.
النتائج: کل الجرذان عاشوا خلال طول فترة التجربة. هذه الدراسة أوضحت ان هناک قلة فى مستوى الکلوتاثایون فی نسیج
الخصیة والکبد فى الجرذان المعاملة مع العلم ان مستوى هذا المضاد التاکسدى فی نسیج الکلى قد کان اعلى من مجموعة
السیطرة. بطریقة مختصرة، الایماتنب احدث زیادة فى مستوى المالوندالدیهاید فى کلا نسیجی الخصیة والکبد بالمقارنة مع
مجموعة السیطرة. ان مستوى المالوندالیهاید فى نسیج الکلى فى مجموعة المعاملة کان اقل من ذلک العائد لمجموعة السیطرة.
الاستنتاجات: ان الایماتنب قد سبب انخفاضا فى مستوى الکلوتاثایون وزیادة فی مستوى المالوندالدیهاید فی مزیجی الخصیة
والکبد ممایدل على تأثیره الاوکسیدى.

Effect of Mobile Phone Radiation on some Hormonal and Biochemical Tests in Blood Serum of Pregnant Rats Rattus norvegicus

Yasir A. Abdullah; Janan H. Abdul-Fattah

Rafidain Journal of Science, 2018, Volume 27, Issue 1, Pages 235-245
DOI: 10.33899/rjs.2018.143932

This study was conducted to investigate the effect of electromagnetic waves of mobile phone on some hormonal and biochemical tests in blood serum of pregnant rats. 60 pregnant rats were used and divided in to 6 groups, including control and five experimental groups: switch off mobile phone for 1 hrday, switch on and connect mobile phone for 12 hr, 1 hr, 2 hr, 3 hrday. The exposing period was 18 days starting from 1st day to 18th day of pregnancy. (5 – 6) ml of blood were drawn from the eye orbital to measure the concentration of estrogen, progesterone, glutathione and malondialdehyde in blood serum.
The results showed that exposing of pregnant rats to electromagnetic waves of mobile phone caused significant lowering of both estrogen and progesterone hormone concentration, and caused significant decreasing of glutathione concentration and significant increasing of malondialdehyde concentration in blood serum in comparison with control group

The Effect of Flaxseeds on some Physiological and Biochemical Characters in Liver Tissue of Quail Birds

Muntaha M. Al-Kattan; Zahra I. D. Bashi; Abeer A. Katheem

Rafidain Journal of Science, 2018, Volume 27, Issue 1, Pages 54-63
DOI: 10.33899/rjs.2018.141072

This study was conducted to investigate the effect of flaxseed on some biochemical characteristics represented by ALT, AST ALP, enzymes, as well as to determine glutathione and malondialdehyde levels and body weight of birds which ranged between (175-200)g, (60-70) days old. Male Quail birds were divided into five groups 5 birds-group.
Flaxseeds were used after grinding in different concentrations as capsules of (250, 500, 1000, 2000) mg/kg of body weight. The birds were treated daily with that flaxseeds powder.
Results showed a good improvement in the biochemical qualities as well as a rise in the level of glutathione and the reduction of MDA level in addition to lowering the body weights of birds, especially in the group given 2000 mg/kg of body weight.
Histological examinations showed no differences in comparison with the control group in birds given 250mg/kg body weight of the flaxseeds while other groups showed pathological changes in liver tissue.

A Biochemical Study of Multiple Types of Honey Bees

Zahra I. Dallal Bashi

Rafidain Journal of Science, 2014, Volume 25, Issue 4, Pages 92-103
DOI: 10.33899/rjs.2014.88663

The present study was performed to detect the biochemical properties of ten types of honey bees (Black Bean, Forests, Rosemary, Thyme, Citrus, Willow, Acacia, Sidr, Pine and Mountain) which were collected from different pasture areas. The level of glutathione, malondialdehyde, activity of acid phosphatase, calcium, glucose, total protein and albumin were measured.
The results showed a significant increase in the glutathione and calcium level and a significant reduction in the malondialdehyde of dark honey samples (honey of forests, pine and Black Bean) compared with other honey types. Honey with more acidity (Citrus and Willow) showed a significant increase in acidic phosphatase compared with other samples. The results also showed a significance increase in glucose level and a significant reduction in the level of total protein and albumin for honey types (Acacia, Rosemary, Willow, Citrus and Thyme) compared with other types (forests, Black Bean, Mountain, pine and Sidr).
In conclusion, honey varies in its components according to the variation in flowers nectar and this leads to a variation in its biochemical components even in a low ratio. In addition to the superiority of the dark honey type (forests, pine and Black Bean) in antioxidants and food value comparison with other light-color honey samples (Citrus, Willow, Acacia and Rosemary).

Effect of Lead on some Antioxidants and Lipid Peroxidation in Blood of White Male Albino Rats

Muna H. Jankeer

Rafidain Journal of Science, 2012, Volume 23, Issue 4, Pages 55-69
DOI: 10.33899/rjs.2012.44411

The present study includes the investigation of the effect of lead on some enzymatic, non enzymatic antioxidants and lipid peroxidation in serum of male albino rats Rattus norvegicus aged 2.5-3 months. Fifty male rats were randomly divided into 5 groups (10 rats/ group).The first group: was given standard forage and distilled water (as a drinking water) and considered as control. The second group: was given standard forage and distilled water (as a drinking water) containing 20 mg lead /L. daily for a period of 15 days. Third group: was given standard forage and distilled water (as a drinking water) containing 40 mg lead/ L. daily for a period of 15 days. The fourth and fifth groups were treated with the same concentration of the second and third group separately daily for a period of 30 days.
The results showed that treatment with lead caused a significant decrease in the concentration of each of total protein, albumin, globulin, glutathione GSH and the superoxide dismutase SOD activity in serum of treated rats compared with the control group. The reduction of the above mentioned parameters was progressive with the time of treatment. The result also showed that treatment with lead caused a significant increase in uric acid and malondialdhyde MDA in serum compared with control group. The results also showed that the decrease and increase were more prominent in rats treated with 40 mg
lead/ L of drinking water. These results suggests that lead may induce oxidative stress in albino rats.

Comparative Effects of Alpha – methrin (Serin) and Actara (Cruzer) Insecticides on some Enzymatic and Non-enzymatic Antioxidants and Lipid Peroxides in Serum and Tissues of White Male Albino Rats

Sarab Y. Al-Qassim; Muna H. Jankeer

Rafidain Journal of Science, 2010, Volume 21, Issue 4, Pages 1-20
DOI: 10.33899/rjs.2010.37414

The present study included the recognition of alpha-methrin (Serin) and actara (Cruzer) insecticides medium lethal dose LD50, which was found to be 150 mg alpha-methrin/Kg body weight and 1875 mg actara / Kg body weight. By consideration of (LD50) values three doses of each insecticide were orally administered to male albino rats for 15 and 30 days period, to study their effects on the levels of some enzymatic, non enzymatic antioxidans and lipid peroxides in serum and tissues of albino rats.
The rats were divided into 7 groups; the control group which administered with distilled water, the first to third group were administered with 2.5, 5, 10 mg /Kg body weight alpha-methrin respectively, the fourth to sixth group were administered with 100, 150, 200 mg /Kg body weight actara respectively on comparison to control group with that treated with either alpha-methrin or actara. The results showed a significant decrease in non enzymatic antioxidant (uric acid concentration and glutathione) level and an increase in the activities of superoxide dismutase (enzymatic antioxidant) and a significant increase in malondialdehyed level in serum blood of rats treated with either alpha-methrin or actara. The results also showed a significant decrease in level of glutathione accompanied with significant increase in malondialdehyed level in tissues of (brain, heart, liver and kidney). The rats which administered with different concentrations of alpha-methrin insecticide, showed more effects than that groups administeared by actara. These results indicated that the alpha-methrin has more poisonous effects than actara insecticide .

Effect of Cement Pollution on some Biochemical Parameters in the Blood Serum of Hamam AL-Alil Cement Factory Workers

Haitham L. Al-Hayali

Rafidain Journal of Science, 2009, Volume 20, Issue 3, Pages 19-28
DOI: 10.33899/rjs.2009.40131

Long time exposure to cement industry pollution leads to several health problems. This study was designed to investigate the effects on 55 workers which were divided into groups according to the departments of Hamam AL-Alil Cement Factory-Iraq. The biochemical parameters included in the study are: calcium, zinc, lactate dehydrogenase, alkaline phosphatase, acid phosphatase, glutathione and malonaldehyde.
The results showed that there was a significant increase in calcium, lactate dehydrogenase, alkaline phosphatase, acid phosphatase and malonaldehyde in workers serum which increased with increasing exposure time. The results also showed a significant decrease in glutathione and zinc in workers serum which increased with increasing exposure time in all factory department.