Keywords : well water

Contamination of Domestic Well Water in Nineveh Governorate with some Pathogenic Bacteria and Detection on its Ability to Biofilm Formation

Amera M. Al-Rawi; Manar F. Altaee

Rafidain Journal of Science, 2019, Volume 28, Issue 3, Pages 90-104
DOI: 10.33899/rjs.2019.163162

Due to the unavailability of the drinking water in Mosul city in the period between                      (2014-2017), people started digging wells in their houses to use it in daily life for consumption and irrigation. Forty well water samples were chosen for this study in living quarters (AL-Dubat, Adan, AL-Mouthana, AL-Noor, AL-Zhoor, AL-Jamiea, AL-Falah, AL-Baker). Some pathogenic bacteria were isolated and identified from well water digged in different areas in the left side of Mosul city and to study reconciliation to human consumption. Results showed that fifty-six bacterial isolates obtained included 21 of Escherichia coli, 10 of Aeromonas hydrophila, 8 of Pseudomonas aeroginosa, 7 of Pseudomonas florescens, and show two species ofKlebsiellabacteria, Klebsiella pneumonia and Klebsiella oxytoca 6 and 4 respectively.
Then the ability of these bacterial isolates to form biofilm was studied by using two methods; Tube method and Congo red method, isolates gave 100% positive results in its ability to biofilm formation by tube method. While by congo red Aeromonas and Pseudomons were unable to form biofilm.
Also the study included determination of antibiotic  sensitivity and resistance of bacterial isolates to different antibiotics as it is considered one of the important virulence factors , Results showed that there is variation in its sensitivity and resistance to these antibiotics.