Keywords : Irradiation

Sterilization of Culture Media for Microorganisms Using a Microwave Oven Instead of Autoclave

Suzan A. Shareef; Payman A. Hamasaeed; Abdulilah S. Ismaeil

Rafidain Journal of Science, 2019, Volume 28, Issue 1, Pages 1-6
DOI: 10.33899/rjs.2019.159390

Different culture media in different volumes were exposed to microwave (MW) irradiation for 2, 3 and 4 minutes to investigate the ability of MW to destroy microorganisms and compared with media sterilized by conventional autoclaving method. MW sterilized media were screened for microbial growth.
Exposure of different microorganisms to microwave irradiation resulted in destruction of all microorganisms within 3 minutes. Using MW for irradiation is a practical, easy, rapid and energy saving way to sterilize different types of culture media with no effects on the quality of culture media and microbial growth after sterilization. It can be used as alternative apparatus instead of autoclave in microbiology laboratories for preparing different sizes in rapid and routine experiments especially in the conditions of the weak electricity current and interruption

Effect of γ- Irradiation on the n- Porous Silicon Structures Prepared by Electrochemical Etching

Abdulkahlig A. Sulaiman

Rafidain Journal of Science, 2018, Volume 27, Issue 2, Pages 173-180
DOI: 10.33899/rjs.2018.145402

Porous Silicon has been prepared by using electrochemical cell at room temperature with etching time (20 min), current (30 mA) and fixed electrolyte solution HF:C2H5OH(1:4). The samples are irradiated by γ-ray with various doses (50,100) Gy. Several techniques such as scanning electron microscope (SEM), X-ray diffraction (XRD) and Ramman Spectrum were used to study the influence of the γ- irradiation of porous silicon (PSi). SEM images show the random distribution of pores that cover all the surface which have different sizes and spherical shapes. XRD analysis, which indicated that n- type porous silicon and the samples irradiated at 50 and 100 Gy of γ- ray grow in hexagonal structure having preferred orientation along (002) plane in c-direction. An extremely symmetric band shape were recognized from Raman spectra of PSi