Keywords : Acid phosphatase


A Biochemical Study of Multiple Types of Honey Bees

Zahra I. Dallal Bashi

Rafidain Journal of Science, 2014, Volume 25, Issue 4, Pages 92-103
DOI: 10.33899/rjs.2014.88663

The present study was performed to detect the biochemical properties of ten types of honey bees (Black Bean, Forests, Rosemary, Thyme, Citrus, Willow, Acacia, Sidr, Pine and Mountain) which were collected from different pasture areas. The level of glutathione, malondialdehyde, activity of acid phosphatase, calcium, glucose, total protein and albumin were measured.
The results showed a significant increase in the glutathione and calcium level and a significant reduction in the malondialdehyde of dark honey samples (honey of forests, pine and Black Bean) compared with other honey types. Honey with more acidity (Citrus and Willow) showed a significant increase in acidic phosphatase compared with other samples. The results also showed a significance increase in glucose level and a significant reduction in the level of total protein and albumin for honey types (Acacia, Rosemary, Willow, Citrus and Thyme) compared with other types (forests, Black Bean, Mountain, pine and Sidr).
In conclusion, honey varies in its components according to the variation in flowers nectar and this leads to a variation in its biochemical components even in a low ratio. In addition to the superiority of the dark honey type (forests, pine and Black Bean) in antioxidants and food value comparison with other light-color honey samples (Citrus, Willow, Acacia and Rosemary).

Study of the Effect of Zinc on the Fertility and Acid Phosphatase Activity and Fructose Concentration in Male Swiss Albino Mice ( Mus Musculus)

Tamara W. Jihad

Rafidain Journal of Science, 2012, Volume 23, Issue 6, Pages 39-54
DOI: 10.33899/rjs.2012.59604

This study was conducted to elucidate the effect of different concentrations of zinc element on male fertility of swiss albino mice by using atomic absorption, and determination of acid phosphatase activity ( Ec.3.1.3.2) compared with control groups. The mice were divided into three groups ( 10 mice / group ) and they were three months old. The first group was treated with 0.5 mg / kg body weight and the second group with 1.5 mg / kg body weight with zinc, while the third group considered as control group. The mice treatment was for 15 and 30 days for the two experiment groups.
The results showed a significant increase ( p≤0.05 ) in weight of testes in the group which treated by concentration 1.5 mg / kg b.w compared with control group. Significant increase (p≤0.05) was also found in testes weight when using the high concentration of zinc compared with the low concentration. Results also showed a significant increase in sperm count of groups treated with zinc as compared with control group. Significant increase (p≤o.o5) in sperm motility was detected in both the first and second groups as compared with control . Significant decrease (p≤0.05) in slugish sperm in group with high concentration of zinc as compared with control. Significant increase in both groups in acid phosphatase activity and the concentration of fructose treated with concentrations 0.5 and 1.5 mg / kg b.w as compared with control.

Alkaline Phosphatase and Acid Phosphatase Activity in Blood Serum, Amniotic Fluid and Placenta of Pregnant Women in Ninavah Governorate

Raja A. Al-Taii

Rafidain Journal of Science, 2011, Volume 22, Issue 2, Pages 68-76
DOI: 10.33899/rjs.2011.32360

The present study included estimation of the alkaline phosphatase and acid phosphatase activity in 60 samples of blood, amniotic fluid and placenta of pregnant women which entered labouring unit in Al-Batol teaching hospital in Ninavah Governorate whose ages between 17-40 years, the data were collected using special questionnaire form.
The results showed a significant increase in the activity of alkaline phosphotase and acid phosphotase in blood serum, amniotic fluid and placenta in both age groups 21-30 and 31-40 years, while there was significant change in age group 17-20 years, compared with control group. The results also showed a significant increase in the enzymes activity in blood serum, amniotic fluid and placenta of hypertensive and diabetic pregnant women compared with control group.