Keywords : cytokines

Diagnosis of some Bacteria Causing Gingivitis in People with Type 2 Diabetes and Investigating some of Their Biochemical and Immunological Parameters

Marwa J. Aljumaily; Najdat B. Mehdi; Sahla KH. Abbas

Rafidain Journal of Science, 2019, Volume 28, Issue 3, Pages 48-54
DOI: 10.33899/rjs.2019.163156

  The study aimed to isolate and identify of the types of bacteria which cause gingivitis. Gram positive bacteria were isolated by 89% more than gram-negative bacteria, 60 isolates were Streptococcus viridans and 5 isolates were Escherichia coli. The biochemical study showed a significant increase in concentration of total cholesterol (P 14≤"> 0.05) in the gingivitis and diabetes groups, as well as in very low density lipoproteins showed a significant increase in gingivitis and diabetes group (P 14≤"> 0.033), High-density lipoproteins showed a significant decrease in the group of gingivitis and diabetes compared to the control group at a significant level (P 14≤"> 0.018). In the immunological parameters, the results showed a significant decrease in the concentration of IL-10 in the group gingivitis and diabetes (P 14≤"> 0.05). The concentration of TNF-α increased in gingivitis and diabetes at a significant level (P 14≤"> 0.05).

Bioinformatics: Effect of Cytomegalovirus Infection on Human Immune Gene Expression

Abdulrhem Th. Al-Ghazal

Rafidain Journal of Science, 2018, Volume 27, Issue 4, Pages 40-50
DOI: 10.33899/rjs.2018.159337

Cytomegaloviruses (CMV) cause common infections in humans with a prevalence of over 70% in adults, reaching 90% in poorer communities and developing countries. In immune-compromised and transplant recipients, CMV causes serious complications including pneumonia and ulcerative colitis. Studying the effect of CMV on human immune-inflammatory genes in immune cellular responses is critically important to explore the molecular mechanisms of viral-host interaction. The aim of this study is to explore changes in the mRNA transcripts of a panel of 84 human inflammatory genes. Ninety blood samples were collected from healthy persons used as control samples and compared with acute infection with CMV sample from bioinformatics data (previous researches). Real time-PCR array were performed to determine mRNA levels expression levels of 84 different cytokines and chemokines in control`s leukocytes concentrate. CMV infection caused upregulation (between 44.99 and 0.92 fold change) in the expression of 13 human immune-inflammatory genes (IL-1 alpha, IL10R Alpha, CXCL10, IL10, CX3CR1, CCL2, CCR5, CCR7, CXCL1, IL-1β, TNF, IFN-alpha 2, CCL5). Gene ontology analysis revealed that CMV causes high impact on four key pathways in infected cells. .