Keywords : Cytomegaloviruses

Prevalence of Cytomegalovirus and its Roles in Cytokines Stimulation in Immunocompromised Patients in Mosul City, Iraq

Nawal Shahir Mahmood; Abdulrhem Thonnon AL-Ghazal

Rafidain Journal of Science, 2020, Volume 29, Issue 3, Pages 17-27
DOI: 10.33899/rjs.2020.166308

Cytomegaloviruses (CMV) cause different infections in humans all over the world with a prevalence exceeds 70% in adults and 90% in poorer communities and developing countries. Two hundred immunocompromised patients (cancer, dialysis, thyroid gland, and thalassemia) infected with CMV were subjected to determine CMV seroprevalence and its effect on cytokines expression using advance molecular test (Real time PCR) and Serological ELISA test.  The results showed high CMV seroprevalence rate in immunocompromised patients but in low rate in thalassemia patients in Mosul city compared with other cities near Iraq. CMV stimulates upregulation in expression (mRNA transcripts) of three cytokines genes (IL-10, TNF, and CCL2) which affect four key immune pathways which in turn may cause severe complication on patients such as asthma and cytokine storm.

Bioinformatics: Effect of Cytomegalovirus Infection on Human Immune Gene Expression

Abdulrhem Th. Al-Ghazal

Rafidain Journal of Science, 2018, Volume 27, Issue 4, Pages 40-50
DOI: 10.33899/rjs.2018.159337

Cytomegaloviruses (CMV) cause common infections in humans with a prevalence of over 70% in adults, reaching 90% in poorer communities and developing countries. In immune-compromised and transplant recipients, CMV causes serious complications including pneumonia and ulcerative colitis. Studying the effect of CMV on human immune-inflammatory genes in immune cellular responses is critically important to explore the molecular mechanisms of viral-host interaction. The aim of this study is to explore changes in the mRNA transcripts of a panel of 84 human inflammatory genes. Ninety blood samples were collected from healthy persons used as control samples and compared with acute infection with CMV sample from bioinformatics data (previous researches). Real time-PCR array were performed to determine mRNA levels expression levels of 84 different cytokines and chemokines in control`s leukocytes concentrate. CMV infection caused upregulation (between 44.99 and 0.92 fold change) in the expression of 13 human immune-inflammatory genes (IL-1 alpha, IL10R Alpha, CXCL10, IL10, CX3CR1, CCL2, CCR5, CCR7, CXCL1, IL-1β, TNF, IFN-alpha 2, CCL5). Gene ontology analysis revealed that CMV causes high impact on four key pathways in infected cells. .