About Journal

Rafidain Journal of Science (RJS) is a  scientific and open access journal  Publishing under the license of Creative Commons Attribution 4.0 International (CC-BY), this journal is published fourth issue by the College of Science, University of Mosul, Iraq, since date of first issue (1976), No. of Issue per year (8) issues till the end of 2012 and (12) issues from 2013, (6) issues in 2014 , (2) issues in 2017. No. of papers per Issue (250 page ). No. of Issues published between 1976-2020 (102). We are using iThenticate to prevent plagiarism and to ensure the originality...
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Isolation and Identification of Candida dubliniensis in Patient with Cervical-Vulvovaginal Candidiasis in Erbil City

Nareen Q. Faqe Abdulla; Hero M. Ismael

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 1-8
DOI: 10.33899/rjs.2022.175386

        Vulvovaginal Candidiasis (VVC) is a fungal infection caused by Candida spp. of the genital mucosa that is still a health concern for women.This is a first-of-its-kind investigation on the isolation of Candida dubliniensis, from cervical-vulvovaginal patients in Erbil hospitals. The study was carried out for isolation and identification which performed by using phenotypic and molecular approaches. A total of one hundred samples of vaginal swabs (Copan transport swabs dry (in a cover)), were taken from patients with cervical vulvovaginal candidiasis in order to test for the presence of Candida species. According to macroscopic and microscopic examination, Candida spp. was discovered in 60 of the 100 samples (60%). On CHROM agar medium, eleven non-duplicated C. dubliniensis were identified depending on color of colony (dark green colonies-blue colony). Then the species were confirmed using API 20C assay. Finally, a multiplex PCR technique was performed depending on two types of primers, which are universal and specific for identifying C. dubliniensis and the results of the CHROM agar and API 20C assays were identical with molecular method.

Synthesis of some New Derivatives of Triazole using Ortho-Carboxybenzaldehyde as a Synthone

Maysaa Kairulden Shakir Al-Mala Khder; moayed Salim noori

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 9-18
DOI: 10.33899/rjs.2022.175388

       In this work, the 1,2,4-triazole ring was prepared by introducing the starting material ortho-carboxybenzaldehyde (1) in the usual esterification reaction, in ethanol as a solvent and in the presence of concentrated sulfuric acid to give ethyl-2-formylbenzoate (2), then introducing the resulting ester in a condensation reaction with thiosemberazide in the presence of ethanol as a solvent to afforded 2-(2-formylbenzoyl)hydrazine-1-carbothioamide (3), which on cyclisation using a solution of sodium hydroxide to obtain the 1,2,4-triazole compound (4), which on treatment with different primary aromatic amines to obtain Schiff bases (5a-e) the prepared Schiff bases were introduced into a reaction with chloroacetyl chloride in the presence of triethylamine as an auxiliary base for the reaction to prepare the tetracyclic azitidinone compounds (beta-lactams) (6a-e) along with the triazole ring in the same compound. Finally, these prepared compounds were characterized by physical and spectroscopic measurements such as melting point, thin layer chromatography (m.p., TLC) infrared spectroscopic measurements FT-IR, and nuclear magnetic resonance spectra  of some compounds1H-NMR.

Synthesis of some new Pyrazoline Carbothioamides and Pyrimidinethiols Derivatives from Bis-α, β-Unsaturated Carbonyl Compounds

Zina K. Yahya; Muneera Y. Roof

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 19-28
DOI: 10.33899/rjs.2022.175389

              In our research, new bis-α, β-unsaturated carbonyl compounds (Z2-Z4) were prepared via Claisen- Schmidt reaction, by the condensation of 2,6-dimethyl-3,5-diacetyl-1,4-dihydropyridine (Z1) with different appropriate benzaldehyde, like (benzaldehyde, m-nitro benzaldehyde and o-chloro benzaldehyde) using 10% aqueous sodium hydroxide as a base.
        A simple and efficient method has been applied for the synthesis 2,6-dimethyl- 3,5-diacetyl-1,4-dihydropyridine (Z1) by multi components Rudolf-Hantzsch condensation reaction which included heating the starting materials (paraformaldehyde, acetylacetone and ammonium acetate) at 80Cͦ degrees centigrade in aqueous medium. Bis-α, β-unsaturated carbonyl compounds (Z2-Z4) can be considered as useful intermediates leading to the formation of several heterocyclic compounds such as pyrazoline carbothioamide (Z5-Z7) and pyrimidinethiole (Z8-Z10). The titled compounds (Z5-Z7) and (Z8-Z10) were prepared by interacting with thiosemicarbazide in neutral medium, and thiourea in basic medium respectively. These mentioned compounds (Z1-Z10) were characterized by Ferrier Translate-Infrared spectrum (FT-IR) and Ultraviolet spectrum (UV), in addition to some physical properties. Also, all these reactions followed by thin layer chromatography (TLC) technique and calculate the retardation factor (Rf) values.

A Simulated Study of 65 nm CMOS 2GHz Front-End Preamplifier Circuit for Optical Fiber Applications

Ruwaida A. Al-Berwari; Muhammed Hameed Alsheikhjader

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 29-41
DOI: 10.33899/rjs.2022.175390

   In this research a new design of the transimpedance amplifier (TIA) with the current mirror was employed by the technique (65nm). The TIA consists of a common gate transistor amplifier (CG TIA) and a common source amplifier as an input stage with local active feedback with a second stage of a current mirror and local active feedback to increase gain. In order to verify the performance of the proposed TIA, a circuit simulation was carried out in the LT spice program using coefficients with the technique (65nm CMOS). The simulation results indicate that the interfacial impedance gain is (41 dBΩ) at a bandwidth frequency of (2.0 GHz-3dB) for an input capacitor of          (100 fF) and an input referred noise current spectral density of (14 pA/√Hz) and a power consumption value of                (0.091 mw) at an applied voltage (1V). The main focus of this research is low consumption of power and voltage compared to another research.

Effect of Three Different Dielectric Substrates on the Performance of Rectangular Microstrip Antenna Designed for 2.4 GHz using CST

Laith Al Taan; MOHAMMED SALIM MOHAMMED WARED SALIM ADO

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 42-54
DOI: 10.33899/rjs.2022.175392

This paper presents a design of a low profile inset feed microstrip rectangular antenna working at 2.4 GHz to support application in industrial, scientific, and medical sectors. The design of the antenna is achieved by using three different substrates with dielectric constants of 2.2, 3.6, and 4.3, each with variable substrate thicknesses of 0.6, 1.5, 1.9mm in order to know the effect of substrate properties on the antenna performance such as gain, efficiency, return loss, and frequency bandwidth. The dimensions and performances of the designed antenna are optimized by using Computer Simulation Technology (CST) software hence a comparison has been performed among previous the antenna performances. The practical results for the antenna with the substrate of (dielectric constant of 4.3) and (thickness of 1.5mm) show that the return loss is (-33) dB which close to the simulation value, but there is a small difference (about ~30MHz) in the Impedance bandwidth. However, all other results were close.

Statistical Analysis of the Monthly Activity of the Solar Flare

Imad Ahmed Al-Ibrahimi

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 55-63
DOI: 10.33899/rjs.2022.175396

The study of solar flares (SF) is significant for a more understanding of the nature and dynamics of the sun as well as its impact on space weather. This paper will present a new idea by studying the monthly activity of SFs by collecting data for SFs, the flare index (FI), and the sunspots number (SSN) for the period from 1986 to 2019, which represents three solar cycles 22, 23 and 24. Extracting the monthly averages of the data shows us that the southern solar hemisphere is more active for solar flares than the northern solar hemisphere. Also, the second half of the year is more active than the first half of the three solar cycles. The most active months are March, (July or October) and November, while February was the least active. In addition, the evidence of the cross-correlation results in a strong positive relationship between the three variables. This study serves to forecast the state of the space weather during the months of the year.

The Role of Sulfa Drugs in our Life

Salim J. Mohammed; Azzam A. AL-Hadedi; Salih A. Abood

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 64-74
DOI: 10.33899/rjs.2022.175395

         Gerhardt Dumac discovered in 1932 that Prontosyl kills bacteria while testing dyes, and in 1934, he began using Prontosyl as a treatment. This was in Germany, and experiments in France showed that the effect of Prontosyl is due to the presence of sulfanilamide in it. In 1908, Gelmo was the first to attend a sulfa drug conference in Germany. Sulfanilamide's medicinal usefulness was endorsed in 1936 by researchers at JohnsHopkinsUniversity in the United States, including Long and Plus, Marshall, and others. Sulfa drugs are used to treat a range of diseases caused by bacteria, with which it has been possible to save countless lives. It is believed that the effect of sulfa is to stop the growth of bacteria, meaning that these drugs prevent the growth and reproduction of bacteria, which creates an opportunity for the body's defensive forces to eliminate them. Humans are currently treated with sulfonamides for specific disorders, such as urinary system infections. However, sulfonamides are more typically seen in veterinary medicine. Therefore we attempted to explain the role and importance of sulfa drugs in our lives because of their widespread use in medical therapy.

Phenotypic and Molecular Investigation of Streptococcus pneumoniae Hemolysin

Roaa Amer Thamer; Amera M. Al-Rawi

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 1-9
DOI: 10.33899/rjs.2022.175398

The current study aimed to detect S.pneumoniae, which is one of the gram-positive bacteria that gives partial α-hemolysis on blood agar media, as well as has the ability to complete blood hemolysis (beta)-hemolysis under anaerobic conditions. Fifty sputum specimens were collected from patients in Ibn sena and Al Salam hospitals in Mosul/ Iraq from august 2021 to March 2022. Five isolates were diagnosed as S.pneumoniae which exhibited an ability to produce hemolysin when phenotypicallyinvestigated. Polymerase chain reaction (PCR) technique was used to detect hlys gene Electrophoresis results showed four bands (80% of isolates) with a molecular size of 296 bp the PCR product were then sequenced.

Efficiency of Zinc Oxide Nanoparticles in Stimulating Germination and Seedling Growth of Soybean Plant Glycine max L. and its Protein Content

Ghada Mahdi; Rana T. Yahya

Rafidain Journal of Science, 2022, Volume 31, Issue 3, Pages 10-17
DOI: 10.33899/rjs.2022.175399

           The results of this study gived a scientific view about the role of zinc oxide nanoparticles (ZnO) in the seeds germination and seedlings growth of soybean (Glycine max L.) when placed on the surface of solid Murashige and Skoog (MS) medium supplemented with concentrations                                   (0, 20, 40, 60, 80, 100µg/ml)  of those nanoparticles as stimulating factors. The results showed that the concentration of 40 μg/ml was superior to the percentage of germination of the growth seeds after 7 days which was 100 % at compared to the MS medium alone (control) which gave a percentage of germination of 75% after 15 days and also encouraged the same concentration to obtain the best length of the stems and roots groups at 10, 12 cm respectively, compared to the average lengths of seedlings growing on the control medium, with average lengths of 6 and 8.5 cm for stems and roots respectively. Also, this stimulation of zinc oxide nanoparticles was reflected on the growth and division parameters of cells through the protein content and DNA and RNA content of seedling cells, which increased with the presence of these nanoparticles, especially at a concentration of 40 μg /ml, as the protein content of cells reached 1.92 μg\ gm and with DNA, RNA contents 12.45 and 99.43 μg /gm respectively, compared to their ratios in seedling cells growing on MS medium.
 

Sepsis in Burn Patients

Ghassan A. Hassan

Rafidain Journal of Science, 2019, Volume 28, Issue 4, Pages 13-21
DOI: 10.33899/rjs.2019.163292

One hundred blood samples and burn swabs from septicemic burn patients were collected over a period from July 2017 until may 2018 at Burn Center in Baghdad Teaching Hospital. The age of patients was between one year to sixty years and they were suffering from burn wound sepsis ,the highest number of patients was female among age group 20-29 years old. The highest frequent cause of burn was flame 78% and the least was chemical 1%, it was found that flame burn patients were more vulnerable to sepsis. The mortality rate among male 56%, females 48% and the highest mortality rate was 100% in the age group less than one year and more than sixty years, the least rate of mortality was 27% in the age group 10-19 years old. The number of septicemic patients 36 who is total body surface area (BSA) burned 30-39% and the mortality in this group was 16.6%, however the least number of patients was two of BSA 90-99% with mortality rate 100%. The most frequent aetiological agents isolated from the blood of septicemic burn patients was staphylococcus aureus 34(28.1) followed by Pseudomonas aeruginosa 25(20.7), Klebsiella pneumonia 13(10.8), Streptococcal pyogenes 10(8.3), Escherichia coli 8(6.6%) and Serratia marcescense, Acinnetobacter calcoceticus were 5(4.1) equally. The incidence of gram negative bacteria was much higher than gram positive bacteria in the septicemic burn cases, most  of septicemic attack occur during the first week of admission and especially on the 7th day of staying in hospital and above, and they were much more  likely to result from gram positive organism e.g S.aureus and S.pyogene. After the 1st week the septicemic attack was much more likely to result from gram negative organisms e.g. P.aeruginosa  K.pneumonia, E.coli, Serratia mercescense, Acintobacter calcoceticus, P vulgaris and P. rettger.

Sterilization of Culture Media for Microorganisms Using a Microwave Oven Instead of Autoclave

Suzan A. Shareef; Payman A. Hamasaeed; Abdulilah S. Ismaeil

Rafidain Journal of Science, 2019, Volume 28, Issue 1, Pages 1-6
DOI: 10.33899/rjs.2019.159390

Different culture media in different volumes were exposed to microwave (MW) irradiation for 2, 3 and 4 minutes to investigate the ability of MW to destroy microorganisms and compared with media sterilized by conventional autoclaving method. MW sterilized media were screened for microbial growth.
Exposure of different microorganisms to microwave irradiation resulted in destruction of all microorganisms within 3 minutes. Using MW for irradiation is a practical, easy, rapid and energy saving way to sterilize different types of culture media with no effects on the quality of culture media and microbial growth after sterilization. It can be used as alternative apparatus instead of autoclave in microbiology laboratories for preparing different sizes in rapid and routine experiments especially in the conditions of the weak electricity current and interruption
.

Spectrophotometric Determination of Paracetamol Using Diazotization Coupling Reaction

Enas S. Thanoon

Rafidain Journal of Science, 2019, Volume 28, Issue 2, Pages 76-83
DOI: 10.33899/rjs.2019.159979

An accurate, simple, and sensitive indirect spectrophotometric method which proposed and developed for the determination of paracetamol in different pharmaceutical preparations. The proposed method was based on acid hydrolysis of PAR to produced p-aminophenol (PAP), PAP was diazotization with nitrite ion to form the corresponding diazonium salt, followed by coupling with histidine reagent in alkaline medium to produced azo dye that showed maximum absorbance at 430 nm. Beer's law was obeyed in the concentration rage of 10-500 µg/20 ml (i.e. 0.5-25 ppm). The molar absorpitivity and Sandell's sensitivity of the dye were. 1.118×104 l.mol-1.cm-1and 0.0135 µg.cm-2 respectively. The method successfully has been applied for the determination of PAR in pure form,and its pharmaceutical preparations (tablets, syrup and injection).

).

Effect of Wet Cupping on Serum Lipids Profile Levels of Hyperlipidemic Patients and Correlation with some Metal Ions

Layla A. Mustafa; Rukzan M. Dawood; Osama M. Al-Sabaawy

Rafidain Journal of Science, 2012, Volume 23, Issue 5, Pages 128-136
DOI: 10.33899/rjs.2012.60009

The present study was conducted to evaluate the effect of cupping therapy on serum lipid profile concentration and correlated to some trace elements (Cu, Zn, and Mn). Thirty one men (35 to 55 years old), with hyperlipidemia and without antihyperlipidemic drug or high energy diet consumption for the duration of the study were subjected to cupping. The serum for total cholesterol, triglyceride, lipoprotein (HDL, LDL) and trace elements concentration was collected from brachial veins, and determined before cupping and then once a week for two weeks after cupping. Patients with hyperlipidemia who subjected for cupping show a significantly decrease (p≤0.05) in total cholesterol, LDL cholesterol, and LDL/HDL ratio by comparison before cupping, while there were no significant differences in serum HDL cholesterol and triglyceride concentrations. Zn concentration and Cu/Zn ratio show a highly significant difference (p<0.001) after cupping and also showed a significant correlation with serum lipids profile after cupping, especially zinc metal.

Spectrophotometric Determination of Sulfadiazine via Diazotization and Coupling Reaction - Application to Pharmaceutical Preparations

Salim A. Mohammed; Haseeb Y. S. Zebary

Rafidain Journal of Science, 2013, Volume 24, Issue 11, Pages 61-73
DOI: 10.33899/rjs.2013.80282

A simple, rapid, accurate and sensitive spectrophotometric method has been developed for the quantitative determination of sulfadiazine (SDz) in both pure and its dosage forms. The method is based on diazotization of primary amine group of sulfadiazine with sodium nitrite and hydrochloric acid followed by coupling with γ-resorsolic acid (2,6-dihydroxybenzoic acid) in alkaline medium of sodium hydroxide to form a yellow coloured azo dye shows a maximum absorption at 458 nm against reagent blank solution. Beer's law is obeyed over the concentration range of 10-300 μg of SDz / 25 ml (0.4-12 ppm) with a determination coefficient (R2=0.9998 ) and molar absorptivity 4.38×104 l.mol-1.cm-1 and a relative error in the range of 0.1- 0.64% and a relative standard deviation from  0.27 to  1.21 % depending on the concentration level of SDz. The method is suitable for the determination of sulfadiazine in the presence of other ingredients that are usually present in dosage forms. The effect of organic solvents on the spectrophotometric properties of the azo dye and the composition of the resulting product have also been worked out and it is found to be 1:2 γ-resorsolic acid: sulfadiazine. The method has been successfully applied to the determination of sulfadiazine in its pharmaceutical preparations ( tablet, and burn cream ).

Isolation and Identification of Uncommon Bacteria from Different Infections with Detection some Virulence Factors

Luai M.I. Al-Douri; Mohammed N. Maaroof

Rafidain Journal of Science, 2018, Volume 27, Issue 4, Pages 321-333
DOI: 10.33899/rjs.2018.159328

A total of 220 samples were collected from different sources of infection from the patients of Tikrit Hospital and Samarra Teaching Hospital who showed symptoms of infection, both sexes and different age groups for the period from April 2017 to January 2018. The results of the isolating showed that the number of samples that showed positive bacterial growth on the used media was 135 samples (61.4%),while 85 samples (38.6%) of total samples did not produce significant bacterial growth, The common bacteria isolates in our study were the highest number of isolates with a total of 111 samples and 82.2%, the uncommon bacterial isolates showed in 24 growth samples (17.8%). The results showed that Morganella morganii had the highest percentage of isolates by 3 isolates (12.5%), followed by Aerococcus urinae, Pseudomonas stutzeri, Micrococcus luteus with isolates (8.2%), for other species, only one isolate (4.2%) was obtained, All isolates showed an absolute sensitivity of 100% for Imipenem and 100% absolute resistance to 10 antibiotics,the results showed that both bacteria Kocuria kristinae, O. anthropi, have three virulence factors, Protease, Urease, β-lactamase, While the bacterial species Sphingomonas paucimobilis, Gemella sanguinis, Aerococcus urinae, Pseudomonas stutzeri, Pseudomonas fluorescens, gave a positive test result for the production of β-lactamase, Hemolysin, while Morganella morganii, Alloiococcus Otitis have two factor, Urease, Β-lactamase. As for the isolates Pantoea agglomerans, Kocuria rosea has shown its ability to produce only one type of β-lactamase β-lactamase.

Sterilization of Culture Media for Microorganisms Using a Microwave Oven Instead of Autoclave

Suzan A. Shareef; Payman A. Hamasaeed; Abdulilah S. Ismaeil

Rafidain Journal of Science, 2019, Volume 28, Issue 1, Pages 1-6
DOI: 10.33899/rjs.2019.159390

Different culture media in different volumes were exposed to microwave (MW) irradiation for 2, 3 and 4 minutes to investigate the ability of MW to destroy microorganisms and compared with media sterilized by conventional autoclaving method. MW sterilized media were screened for microbial growth.
Exposure of different microorganisms to microwave irradiation resulted in destruction of all microorganisms within 3 minutes. Using MW for irradiation is a practical, easy, rapid and energy saving way to sterilize different types of culture media with no effects on the quality of culture media and microbial growth after sterilization. It can be used as alternative apparatus instead of autoclave in microbiology laboratories for preparing different sizes in rapid and routine experiments especially in the conditions of the weak electricity current and interruption
.

Estimation of the Concentrations of some Heavy Metals in Water and Sediments of Tigris River in Mosul City

Eman S. Al-Sarraj; Muna H. Jankeer; Sati M. Al-Rawi

Rafidain Journal of Science, 2019, Volume 28, Issue 1, Pages 1-10
DOI: 10.33899/rjs.2019.159401

Tigris river is considered among the most important sources of water in Iraq. Therefore, an ecological study has been conducted on this river within a stretch in Mosul city starting from Mushirfa site north till Al-Busaif village in the south. The study aimed at verifying the impact of various pollutants including heavy metals on water and sediments. The study started on Spring 2011 through Winter 2012.
The heavy metals have been extracted by stander method using Atomic Absorption Spectrophotometer and the estimation of some heavy metal concentrations such as (Zn, Cu, Cd, and Pb) in river water and sediments has been done.
The result reveled a significant increase in the concentration of studied heavy metals in water and sediments in the area of Al-Busaif compared with Mushirfa area (as a control group). The concentration of heavy metal in water followed the descending order: Zn< Cu< Pb .

Contamination of Domestic Well Water in Nineveh Governorate with some Pathogenic Bacteria and Detection on its Ability to Biofilm Formation

Amera M. Al-Rawi; Manar F. Altaee

Rafidain Journal of Science, 2019, Volume 28, Issue 3, Pages 90-104
DOI: 10.33899/rjs.2019.163162

Due to the unavailability of the drinking water in Mosul city in the period between                      (2014-2017), people started digging wells in their houses to use it in daily life for consumption and irrigation. Forty well water samples were chosen for this study in living quarters (AL-Dubat, Adan, AL-Mouthana, AL-Noor, AL-Zhoor, AL-Jamiea, AL-Falah, AL-Baker). Some pathogenic bacteria were isolated and identified from well water digged in different areas in the left side of Mosul city and to study reconciliation to human consumption. Results showed that fifty-six bacterial isolates obtained included 21 of Escherichia coli, 10 of Aeromonas hydrophila, 8 of Pseudomonas aeroginosa, 7 of Pseudomonas florescens, and show two species ofKlebsiellabacteria, Klebsiella pneumonia and Klebsiella oxytoca 6 and 4 respectively.
Then the ability of these bacterial isolates to form biofilm was studied by using two methods; Tube method and Congo red method, isolates gave 100% positive results in its ability to biofilm formation by tube method. While by congo red Aeromonas and Pseudomons were unable to form biofilm.
Also the study included determination of antibiotic  sensitivity and resistance of bacterial isolates to different antibiotics as it is considered one of the important virulence factors , Results showed that there is variation in its sensitivity and resistance to these antibiotics.

Detection of the Efficacy of some Transport, Isolation and Culture Media for Oral Treponema Isolated from Periodontitis

Amera M. Al-Rawi; Summaya A. Muhammad

Rafidain Journal of Science, 2011, Volume 22, Issue 4, Pages 49-67
DOI: 10.33899/rjs.2011.31499

The ability of three types of transport media (Tris-HCl— EDTA fluid , Normal saline and Reduced transport media) to transfer oral treponema samples was investigated , in addition to the capability of relying five types of nutritional media (New oral spirochetes, Supplumented pleupneumolike organism, Thioglycolate medium, Pepton- yeast extract- glucose, and Trypton- yeast extract- glucose- volatile fatty acid-serum medium) and two types of solidified culture media (Thioglycolate-BHI agar and Trypton- yeast extract- glucose- volatile fatty acid-serum agar) for the primary isolation and subculturing of these organisms reaching the most necessary supplements required to support the growth of these organisms. The efficacy of the three types of transport media in transport and maintenance of the vaibilty of these organisms was shown, also the efficacy of primary isolation and subculturing media to support the organisms' growth was proved after supplementing these media with the necessary growth elements. Hence it was possible to provide the optimal anaerobic conditions for the growth by the addition of reducing agents (Sodium thioglycolate and L-cystein) to the culture media and incubation in anaerobic jar, furthermore, introducing the necessary elements of long chain fatty acid by the addition of isobutyric acid and serum, and we confirm the ability of three types of serum (Rabbit serum, Fetal calf serum and Fetal bovin serum) to support the culture media. We had showing possibility of depending upon the selection isolation method by the addition the antibiotic rifampicin. Moreover, it was observed two forms of growth of these organisms: the turbidity after two days of inocubation and the sediment form with the shot silk after four days of incubation, and the variation in colonies form on the solid media was also clear.

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