About Journal

A Scientific and evaluated journal published by the college of Science, University of Mosul, Date of first issue (1976) No. of Issue per year (4)issues till the end of 2012 and 6 issue from 2013. No. of paper per Issue (250 page ) No. of Issue published between 1976-2012 (69)
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Journal Information

Publisher: Mosul University- college of Science

Email:  rafjoursci@alrafidensciencejournal.com

Editor-in-chief: Professor Dr. Amera M. Al-Rawi

Managing Editor: Laith Ahmed Najam

ISSN: 1608-9391

Clinical Study of Rhodanese Enzyme in Renal Failure Patients

Rafidain Journal of Science, Volume 28, Issue 4, Pages 1-7

The research involved clinical study of rhodanese enzyme and its relation with some biochemical parameters in renal failure.
The result demonstrated a significant increase in the activity of rhodanese and metallo-endopeptidase and in concentration of urea, and potassium while a significant decrease activity of sulfate oxidase, thiosulfate oxidase and arylesterase and in concentration of albumin and sodium in renal failure patients compared with control.
The results also demonstrated a significant decrease in the activity of rhodanese in male compared with female in each of patients and control groups.
Correlation coefficients between rhodanese and biochemical parameters of renal failure patients showed that activity of rhodanese has negative correlation with activity of sulfate oxidase, thiosulfate oxidase and concentration of albumin while appositive correlation with activity of metallo –endopeptidase and concentration of urea in renal failure patients.
These results provide evidence of a major role for rhodanese enzyme in renal failure, which led to consider it as a marker for renal failure and its relation with biochemical parameters in patients group.
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Assessing the Environmental Situation for Excretion Management Stations in Al – Qayyarah Refinery

Rafidain Journal of Science, Volume 28, Issue 4, Pages 8-22

The study was accomplished for the evaluation of the efficiency of the processing station of excretion in Al – Qayyarah refinery, which is located in the area of Al – Qayyarah town toward the south east direction of Mosul city / Iraq. For this purpose, monthly samples were collected for five months from November 2013 to February 2014. Physical, chemical and biological tests were carried out on the samples including Electrical Conductivity, Total Dissolved Solid, Turbidity, Hardness, pH, Sulphate, Sodium and Potassium ions, Heavy metals, Oil,Chemical Oxygen Demandand the Total number of bacteria. The results of this study showed that there was clear variations in the values of those variables before and after treatment,such that values of the acidic function before treatment ranged between (7-8.1) compared with the value after treatment which ranged between (6.9-8.2).the values of Electrical Conductivity was (460-720)µmhos/cm before treatment while the values after treatment was(350-630)µmhos/cm the rate of total hardness before treatment was (233.8) mg/L compared with it’s rate after treatment (238.6) mg/L the nitrates ranged between(0.403-1.341)mg/L before treatment and (0.470-1.423) mg/L after treatment and for sulphate the values ranged between (56.16-67.416) mg/L before treatment compared with values after treatment which was (55.33-69.83) mg/L, the values of concentration for the heavy metals(Pb, Co, Cd, Cu) before treatment was (1.536-1.627), (0.05793-0.06644), (0.04794-0.04821), (0.5378-0.5733) mg/L and thevalues after treatment was (1.324-1.410), (0.06899-0.07814), (0.04845-0.06227), (0.5623-0.5825) mg/L and the total number of bacteria was between (100-1100) cell/ ml before treatment comparedwithresults after treatment (300-3000) cell/ml. The results show that the station’s pollutants removal was not efficient.

Genetical Analysis of Self-Fertilized Generation Variances in Two Durum Wheat Crosses

Nabeel T. Al-Badrany; Najeeb K. Yousif; Ghada A. Al-Hamdany

Rafidain Journal of Science, Volume 23, Issue 6, Pages 68-82

Phenotypic variance in parents and third generation were analyzed to estimate the additive genetic variance, dominance genetic variance, environmental variance, average degree of dominance, heritability in broad sense, heritability in narrow sense and expected genetic advance from selection in F3 generation, of two crosses in durum wheat (Triticum durum Desf.), the first cross between Azeghar-1 and Um-Rabie-5 and the second cross between Leeds and Waha for traits, heading time, flag leaf venation, plant height, peduncle length, number of spike, spike length, grain yield, 100 grains weight and number of grains per spike. Average degree of dominance revealed the different types of dominance for the studied traits, the values of narrow sense heritability were high for plant height, peduncle length, number of spikes, grain yield and number of grains per spikes in both crosses, spike length and weight of 100 grains in first cross, and flag leaf venation in second cross.

The Applications of Al- Chrome Azurol S Color Reaction to Assay Mishraq Alum Purity and Fluoride in Tablet

Hanan H. Ahmad; Farha K. Omar

Rafidain Journal of Science, Volume 22, Issue 6, Pages 60-72

In the present research, a spectrophotometric method is suggested as an alternative to the gravimetric method used to find mishraq alum purity. The method is based on the complex formation between aluminium ion and either Chrome Azurol S (CAS) or Eriochrome cyanine R (ECR) to give colored products suitable for the determination of aluminium. The same reaction has been applied to the determination of fluoride in tablet, depending on the bleaching effect of fluoride on the formed aluminium colored complex. Statistical comparison of method has given satisfactory results

Spectrophotometric Determination of Cobalt(II) with Mordant Blue 9 -Application to Vitamin B12 (Injections and Powder)

Saddalah T. Sulaiman; Tamathir A. Hamoudi

Rafidain Journal of Science, Volume 27, Issue 5, Pages 93-102

A spectrophotometric method has been developed for determination of cobalt with Mordant Blue 9 in presence of cetyltrimethylammonium bromide (CTAB) in order to form an intense violet-colored chelate which exhibits maximum absorption at 586 nm at pH 3. Beer’s law is obeyed over the range of 0.004 -1 ppm with a molar absorptivity 3.97×104 l.mol-1.cm-1, Sandell’s sensitivity index of 1.484 ng.cm-2, while LOD (limit of dedication), LOQ (limit of quantitation) are found to be 4.006 ng/ml (n=10) and 13.353 ng/ml (n=10), respectively. The method has been applied for determination of Co(II) in pharmaceutical preparations.

Bacteriological Study of some Locally Prepared Salads in some Restaurants in Mosul City

May A. Al-Allaf; Amera M. Al-Rawi; Abdul Muhsin S. Shehab

Rafidain Journal of Science, Volume 25, Issue 4, Pages 70-80

The Bacterial contamination of some locally prepared salads in some restaurants in Mosul city was studied. These salads were prepared from vegetables and legumes with souse and flavors. Samples were collected from random restaurants in four different places in Mosul city including: Al-Majmoa Al-Thakafia, Al-Zuhoor, Al-Nabi Younis and Al-Dawasa.
Total plate count, gram negative and spore forming bacteria were detected, as well as some pathogenic bacteria such as Yersinia enterocolitica, Listeria monocytogenes and Staphylococcus aureus.
In general, the results showed contamination of these foods with variable numbers and rates of different types of bacteria in these restaurants under study which comprised harmful on environment and general health.
Al-Majmoa Al-Thakafia restaurants were showed lowest rate of contamination with Yersinia enterocolitica, Listeria monocytogenes and Staphylococcus aureus in cucumber and tomato salad (0%) and in spakety with (0,5,15%), and the percentages of contamination were different in miyones and hommus betahena, while salads collected from Al-Nabi Younis restaurants showed high contamination rate with those three pathogenic bacteria as hommus betahena represent the highest rate of contamination (40,30,60%) for each of Yersinia enterocolitica, Listeria monocytogenes and Staphylococcus aureus respectively.

Association Equilibria of Co(II) and Cu(II) Complexes of Salicylidine p-chloroaniline in Different Solvents

Amel G. Abed; Ahlam M. Jameil; Banan A. Akrawi

Rafidain Journal of Science, Volume 24, Issue 1, Pages 55-69

The electrical conductivity of cobalt salicylidiene chloroaniline and cupper salicylidiene chloroaniline in water, methanol, ethanol, isopropanol and mixture of water-methanol with different composition (100-20%) were measured at 303K. The molar conductivity of the complexes were treated by Lee-Wheaton equation and Kohlrausch equations. The limiting molar conductivity ( ), ( ), the ionic association constant (KA) as well as the main distance between cations and anions (R) were measured. Generally, it was found from the analysis of the results that ( ) decreases with decreasing the variation of dielectric constant of the solvents as well as decreasing the percentage of water. The association constants of the two complexes were increased with decreasing the dielectric constants and increasing of viscosity. The distance parameter (R) indicated that the ions existed as solvent separated ion-pairs (SSIP).

Determination of Transposition Property of Neomycin , Streptomycin and Trimethprim Resistance Genes in Clinical Isolates of Escherichia coli and Proteus mirabilis

Khalid D. Ahmed

Rafidain Journal of Science, Volume 17, Issue 11, Pages 8-15

Twenty bacterial isolates were collected from human diarrheal samples and then identified. Seven out of twenty isolates were E. coli and three P. mirabilis . These isolates were checked for their resistance to six antibiotics and two heavy metals ( HgCl2 and Cdcl2). The bacterial isolates showed variation in their resistance to antibiotics and heavy metals.and only one E. coli but all P. mirabilis isolates showed high level of resistance. Transposition induction of genes in the two chosen bacterial isolates was carried out.. Our results suggested that in E. coli isolate, neomycin resistance gene was induced to jump and insert itself into chloramphenicol and tetracyclin resistance genes causing them to mutate .in percents reaching 80% and 8% respectively. On the other hand, the streptomycin resistance gene transposition appear to be specific for generating mutations in the heavy metals resistance genes at rates 48% and 100 % respectively. In addition, transposition induction of the above genes in the chosen P. mirbilis isolate seemed to cause mutations in ampicilin, chloramphenicol and tetracyclin resistance genes ranging between 2 and 4 % .

Isolation and Identification Species of Vibrio Genus from Fresh and Frozen Shrimp and Confirmation the Identification of Vibrio parahaemolyticus by Polymerase Chain Reaction Technique

Rafidain Journal of Science, Volume 27, Issue 7, Pages 62-76

This research was concerned with the isolation and identification of Vibrio parahaemolyticus from seafood (shrimp) samples. (40) samples of freshly harvested shrimp from the local markets in Al-Basra city and twenty (20) samples of frozen shrimp (Turkey origin) were collected. Two enrichment methods were used for the enhancement of the growth and isolation of vibrio species, which include enrichment in non selective medium Alkaline peptone water (APW) for 8 hours and then in selective broth salt polymyxin broth (SPB) and then plated on two solid selective media, ThioSulphate Citrate Bile Sucrose agar (TCBS) and Arabinose Ammonium Sulphate Cholate agar (AASC) and morphological, cultural and biochemical tests were performed on the colonies grown on selective media, then we confirmed the identification by using the chromogenic medium ChromagarTM Vibrio for the first time locally. Finally tox R based polymerase chain reaction was used to cofirm the identification of V. parahaemolyticus. The results showed the isolation of Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus, Vibrio mimicus in percentage 45%, 20%, 37.5%, 17.5% from fresh shrimp, 35%, 20%, 25%, 15% from the frozen shrimp respectively. The results also showed that there is an accordenance between biochemical tests and ChromagarTM Vibrio. The results of tox R based PCR revealed that 22l25 of V. parahaemolyticus contain the tox R gene which is species-specific gene.

Determination of the Concentration of 40K of Mudstone in Northern Iraq by Using Gamma Ray Spectroscopy and Flame Photometry Technique

Hala D. Kharrufa

Rafidain Journal of Science, Volume 24, Issue 5, Pages 97-107

The study of natural radionuclide materials is important to assess the health hazards on human being and one of these materials is 40 K which is present in soil and different types of stones. This study is performed to assess the 40K concentration in mudstones in the north of Iraq by (flame photometry and γ-ray spectroscopy) and to compare between these two techniques in measuring its level. So the activity of potassium was measured by these methods, and their results were compared with the results of other studies. The results show that the concentration of 40K ranges from 284.3 Bq/kg to 2481.2 Bq/kg using γ-ray spectroscopy and 511.7 Bq/kg to 3623.6 Bq/kg using flame photometer technique and these results were comparable with the upper level of the world mean specific concentration of 40K and cause no health hazard. The results obtained by the two methods were also, compared using T-test and P-value and they show no statistical significant difference.

Hydraulic Evaluation Of The Water Treatment Plants In Baghdad City

Lilian Y. M. Al. Saka; Adnan A. Al. Samawi

Rafidain Journal of Science, Volume 16, Issue 5, Pages 35-42

This study is an attempt to evaluate the suitability of the water supply plants of the city of Baghdad, These plants are: A, B, C, D, E, F and G. Important topographical and hydraulic parameters, such as; location of water plant site relative to river (upstream or downstream), type of river section (on straight reach or on bend reach), location of plant site relative to river bank (west side of river or east side of river), type of intake (good or poor), were investigated to assess the operating situation of each water supply station.
The results of the study indicated that the water supply plant is the most suitable one according to the investigated parameters .The Al Karama water supply plant was found to be the most unsuitable one. It is recommended that such plant should be put out of service.

Isolation of Colour and Resistant Mutants in Alternaria alternata

Huda W. Hadi; Sahi J. Dhahi

Rafidain Journal of Science, Volume 23, Issue 8, Pages 1-11

A total of ( 31) white (W) and/or resistant to potassium chlorate (Chl) or sodium selenate (Sel) mutants were isolated from strain AA1; a black wild type strain of the plant pathogenic fungus Alternaria alternata. Exposing conidia of this stain to 20 min UV at a 20 cm – distance was found to kill more than 90% of the conidia and these conditions were adopted to isolate various mutants.Three white mutants; SW1, SW2 and SW3 were isolated as white sectors after two days of irradiating two-day old colonies of AA1. Concentrations of 100, 60, 100 and 80mM potassium chlorate or concentrations of 200, 280, 280 and 200mM sodium selenate completely inhibited the growth of strains AA1, SW1, SW2 and SW3 respectively and these concentrations were used to isolate the various resistant mutants. Twenty spontaneous chlorate- resistant mutants were isolated from strain AA1(Chl5-Chl9) and strain SW3(Chl13-Chl27). Five Sel mutants (Sel1-Sel5) and one chlorate resistant (Chl10) mutants were isolated from irradiating conidia of SW2 and AA1respectively. The double mutants SW4, white conidia and chlorate resistant, was isolated by irradiating conidia of Chl10. This collection of mutants should allow starting the genetic analysis of this important fungus.

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